Compositions and methods for rapid detection of
The present invention features rapid and accurate methods for detecting Salmonella (e.g., in a food product, environmental sample, biological sample or other material).
1. A primer selected from the group consisting of a forward primer having the nucleic acid sequence:
(SEQ ID NO: 3)
5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]
[mC][mA]-3′
and a reverse primer having the nucleic acid sequence:
(SEQ ID NO: 4 )
5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]
[mG][mA][mA][mC]-3′,
wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide.
2. A pair of primers comprising a forward primer having the nucleic acid sequence:
(SEQ ID NO: 3)
5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]
[mC][mA]-3′
and a reverse primer having the nucleic acid sequence:
(SEQ ID NO: 4 )
5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]
[mG][mA][mA][mC]-3′,
wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide.
3. A probe comprising the nucleic acid sequence:
(SEQ ID NO: 5)
5′-CGCCTGTGAACTTTATTGGCG-3′.
4. A combination of primers and probe, wherein the primers comprise the following sequences
(SEQ ID NO: 3)
5′-GACTCGATATCGAGTCTTTCCACmCGAAATACmCmGmCmCmA-3′
(SEQ ID NO: 4)
5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]
[mG][mA][mA][mC]-3′,
wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide,
and a detectable probe comprising the following sequence:
(SEQ ID NO: 5)
5′-CGCCTGTGAACTTTATTGGCG-3′.
5. A kit comprising a nicking enzyme, dNTPs, a polymerase, a forward primer having the nucleic acid sequence:
(SEQ ID NO: 3)
5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]
[mC][mA]-3′
and a reverse primer having the nucleic acid sequence:
(SEQ ID NO: 4 )
5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]
[mG][mA][mA][mC]-3′,
wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide,
and a detectable probe comprising the following sequence:
(SEQ ID NO: 5)
5′-CGCCTGTGAACTTTATTGGCG-3′.