IP Library Granted Patent US 9,845,510
Granted Patent B2
US 9,845,510 · App. 14/997,274 · Granted Dec 19, 2017

Compositions and methods for rapid detection of

Inventors: Lars E. Peters (Portland, ME); Vikrant Dutta (Portland, ME); Thomas Guerrette (Portland, ME); Stephen A. Judice (Portland, ME); Breck O. Parker (Portland, ME)
Assignee: ENVIROLOGIX INC.
C12Q1/689
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Quick Facts
Patent No.
US 9,845,510
App. No.
14/997,274
Granted
Dec 19, 2017
Kind
B2
Abstract

The present invention features rapid and accurate methods for detecting Salmonella (e.g., in a food product, environmental sample, biological sample or other material).

Claims (43)

1. A primer selected from the group consisting of a forward primer having the nucleic acid sequence:

(SEQ ID NO: 3)

5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]

[mC][mA]-3′

and a reverse primer having the nucleic acid sequence:

(SEQ ID NO: 4 )

5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]

[mG][mA][mA][mC]-3′,

wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide.

2. A pair of primers comprising a forward primer having the nucleic acid sequence:

(SEQ ID NO: 3)

5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]

[mC][mA]-3′

and a reverse primer having the nucleic acid sequence:

(SEQ ID NO: 4 )

5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]

[mG][mA][mA][mC]-3′,

wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide.

3. A probe comprising the nucleic acid sequence:

(SEQ ID NO: 5)

5′-CGCCTGTGAACTTTATTGGCG-3′.

4. A combination of primers and probe, wherein the primers comprise the following sequences

(SEQ ID NO: 3)

5′-GACTCGATATCGAGTCTTTCCACmCGAAATACmCmGmCmCmA-3′

(SEQ ID NO: 4)

5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]

[mG][mA][mA][mC]-3′,

wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide,

and a detectable probe comprising the following sequence:

(SEQ ID NO: 5)

5′-CGCCTGTGAACTTTATTGGCG-3′.

5. A kit comprising a nicking enzyme, dNTPs, a polymerase, a forward primer having the nucleic acid sequence:

(SEQ ID NO: 3)

5′-GACTCGATATCGAGTCTTTCCAC[mC]GAAATAC[mC][mG][mC]

[mC][mA]-3′

and a reverse primer having the nucleic acid sequence:

(SEQ ID NO: 4 )

5′-GACTCGATATCGAGTCTTTCCGG[mC]ATCATTATTATCTTTG[mU]

[mG][mA][mA][mC]-3′,

wherein “m” preceding a nucleotide indicates the position of a 2′ modification on the nucleotide,

and a detectable probe comprising the following sequence:

(SEQ ID NO: 5)

5′-CGCCTGTGAACTTTATTGGCG-3′.

Assignments (3)
SECURITY INTEREST Recorded Sep 5, 2025
From: ENSIGN-BICKFORD INDUSTRIES, INC.; APPLIED FOOD BIOTECHNOLOGY, INC.; EB ANALYTICS, INC.; ENSIGN-BICKFORD AEROSPACE & DEFENSE COMPANY; ENVIROLOGIX INC.
To: U.S. BANK NATIONAL ASSOCIATION
Reel/Frame 072815/0001 →
SECURITY INTEREST Recorded Feb 4, 2021
From: ENSIGN-BICKFORD INDUSTRIES, INC.; APPLIED FOOD BIOTECHNOLOGY, INC.; ENSIGN-BICKFORD AEROSPACE & DEFENSE COMPANY; EB ANALYTICS, INC.; ENVIROLOGIX INC.; HONEYBEE ROBOTICS, LTD.
To: U.S. BANK NATIONAL ASSOCIATION
Reel/Frame 055223/0048 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 27, 2016
From: PETERS, LARS E.; DUTTA, VIKRANT; GUERRETTE, THOMAS; JUDICE, STEPHEN A.; PARKER, BRECK O.
To: ENVIROLOGIX INC.
Reel/Frame 037598/0301 →
Continuity (3)
Continuation PCTUS2015027036 · Apr 22, 2015
Provisional Application 62110268 · Jan 30, 2015
Related Publication 20160222440A1 · Aug 4, 2016