Molecular markers linked to disease resistance in soybean
The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having a Rps1 resistance allele and resistance to Phytophthora sojae . A soybean plant, part thereof and/or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided.
1. A method of producing a Glycine max plant that has improved resistance to Phytophthora sojae as compared to a control plant, the method comprising the steps of:
(a) isolating a nucleic acid from a Glycine max plant;
(b) detecting in the nucleic acid of (a) the presence of a genetic marker that is associated with improved resistance to Phytophthora sojae and is associated with Rps1-k, wherein the genetic marker comprises SY2725AQ (SEQ ID NO: 8) and further comprises an A allele at nucleotide 251 of SEQ ID NO:8, or is a marker closely linked thereto on Soybean Chromosome 3, wherein the marker closely linked thereto comprises at least one of an A allele at nucleotide 251 of SEQ ID NO:6 (SY2724BQ) or an A allele at nucleotide 251 of SEQ ID NO:7 (SY2724CQ);
(c) selecting a first Glycine max plant based on the presence of the genetic marker detected in (b);
(d) crossing a second Glycine max plant with the first Glycine max plant of (c) wherein, said second Glycine max plant does not comprise in its genome the genetic marker detected in (b); and
(e) producing seed from the crossing of (d) and thereby producing a Glycine max plant that has improved resistance to Phytophthora sojae as compared to a control plant.
2. The method of claim 1 , wherein the detecting comprises detecting at least one allelic form of a polymorphic simple sequence repeat (SSR) or a single nucleotide polymorphism (SNP).
3. The method of claim 1 , wherein the second Glycine max plant is an elite soybean line.
4. The method of claim 1 further comprising the step of backcrossing the plants produced in (e).
5. The method of claim 1 , wherein detecting includes detecting the genetic marker comprising the A allele at nucleotide 251 of SEQ ID NO:8.
6. The method of claim 5 , wherein detecting includes detecting both of the A allele at nucleotide 251 of SEQ ID NO:6 (SY2724BQ) and the A allele at nucleotide 251 of SEQ ID NO:7 (SY2724CQ).