Protein rich food ingredient from biomass and methods of production
The present invention provides a protein material and food ingredient from a sustainable and stable source. The sustainable and stable source of the food or food ingredient is cellular biomass, for example an algal or microbial biomass. The invention discloses that the cellular biomass can be subjected to a series of steps to derive the protein material and food or food ingredient, which has high nutritional content and has pleasing organoleptic properties.
1. A method of making a food product comprising:
producing a protein composition for use in a food product by a method comprising
a) performing a pasteurization step on a cellular biomass comprising cells from an organism of the Class Labyrinthulomycetes prior to lysing of the cells to produce a pasteurized biomass,
b) subsequent to step a), subjecting the pasteurized biomass to an acid wash at a pH of less than 4.5 for at least 10 minutes, wherein the acid wash frees lipid moieties from protein in the cellular biomass; lysing the cells of the biomass; and producing the protein composition for use in a food product; and
combining said protein composition with a food or food ingredient to make said food product;
wherein the protein composition has a protein content of at least 60% w/w, and
wherein the protein composition has a para-anisidine test (pAV) value of less than about 2.0.
2. The method of claim 1 wherein the food product is selected from the group consisting of: a cereal, a snack bar, a soup or stew, a nutrition bar, a binder for bulk artificial meats, an artificial cheese.
3. The method of claim 1 wherein the organism of the Class Labyrinthulomycetes is from a genus selected from the group consisting of: Aurantiochytrium, Botryochytrium, Japanochytrium, Oblongochytrium, Schizochytrium , and Thraustochytrium.
4. The method of claim 3 wherein the protein composition has a protein content of at least 70% w/w.
5. The method of claim 1 , wherein the protein composition for use in a food product is organoleptically acceptable.
6. The method of claim 1 , further comprising performing a delipidation step on the cellular biomass after the pasteurization step and before the cellular biomass is subjected to the acid wash.