IP Library › Patent Application 15012229
Patent Application
App. No. 15/012,229

ALPHA-AMYLASE MUTANTS

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Quick Facts
Patent No.
US None
App. No.
15/012,229
Abstract

The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca 2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.

Claims (22)

1 . A variant alpha-amylase of a parent alpha-amylase, wherein the variant has alpha-amylase activity, and at least 95% homology with the amino acid sequence shown in SEQ ID. NO. 3 comprising one or more of the following substitutions (using SEQ ID NO: 3 for numbering):

I181A,R,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y, or V, wherein the variant has increased specific activity relative to the parent alpha-amylase, wherein specific activity is determined at a temperature of 10° C. to 60° C.

2 . The variant of claim 1 , wherein the variant comprises a substitution of I181A.

3 . The variant of claim 1 , wherein the variant comprises a substitution of I181R.

4 . The variant of claim 1 , wherein the variant comprises a substitution of I181N.

5 . The variant of claim 1 , wherein the variant comprises a substitution of I181C.

6 . The variant of claim 1 , wherein the variant comprises a substitution of I181E.

7 . The variant of claim 1 , wherein the variant comprises a substitution of I181Q.

8 . The variant of claim 1 , wherein the variant comprises a substitution of I181G.

9 . The variant of claim 1 , wherein the variant comprises a substitution of I181H.

10 . The variant of claim 1 , wherein the variant comprises a substitution of I181L.

11 . The variant of claim 1 , wherein the variant comprises a substitution of I181K.

12 . The variant of claim 1 , wherein the variant comprises a substitution of I181M.

13 . The variant of claim 1 , wherein the variant comprises a substitution of I181F.

14 . The variant of claim 1 , wherein the variant comprises a substitution of I181P.

15 . The variant of claim 1 , wherein the variant comprises a substitution of I181S.

16 . The variant of claim 1 , wherein the variant comprises a substitution of I181T.

17 . The variant of claim 1 , wherein the variant comprises a substitution of I181W.

18 . The variant of claim 1 , wherein the variant comprises a substitution of I181Y.

19 . The variant of claim 1 , wherein the variant comprises a substitution of I181V.

20 . A variant alpha-amylase of a parent alpha-amylase, which variant has alpha-amylase activity and at least 95% homology with the amino acid sequence shown in SEQ ID. NO. 3, comprising a substitution (using SEQ ID NO: 3 for numbering):

at position 181, wherein the variant has increased specific activity relative to the parent alpha-amylase, wherein the specific activity is determined at a temperature of 20° C. to 50° C.