Systems and methods for multi-component detection
View Patent ↗A detection system for detecting analytes in a sample, and methods of making and using the detection system, are disclosed. The detection system includes at least one sensor having a substrate and an optical interference layer on the substrate. The optical interference layer includes a plurality of sample segments, wherein two or more of the plurality of sample segments are configured to have different affinities to two or more analytes in the sample. The detection system further includes at least one light source configured to illuminate the two or more of the plurality of sample segments, and at least one image detector configured to detect an interference spectrum from each of the two or more of the plurality of sample segments illuminated by the light source, thereby detecting the two or more analytes in the sample.
1. A detection system for detecting two or more analytes in a sample, the system comprising:
at least one sensor comprising:
a substrate; and
an optical interference layer on the substrate, the optical interference layer comprising a plurality of sample segments, wherein two or more of the plurality of sample segments are configured to have different affinities to two or more analytes in the sample, wherein the optical interference layer comprises TiO 2 , ZrO 2 , HfO 2 , Cr 2 O 3 , or any combination thereof, and wherein the optical interference layer has a porous surface;
at least one light source configured to illuminate the two or more of the plurality of sample segments; and
at least one image detector configured to detect an interference spectrum from each of the two or more of the plurality of sample segments illuminated by the light source, thereby detecting the two or more analytes in the sample.
2. The detection system of claim 1 , wherein the light source is configured to emit ultraviolet light, visible light, infrared light, or any combination thereof.
3. The detection system of claim 1 , wherein the light source is a broadband light source.
4. The detection system of claim 1 , wherein the substrate comprises a metallic material selected from titanium (Ti), silicon (Si), aluminum (Al), germanium (Ge), zirconium (Zr), hafnium (Hf), rutherfordium (Rf), chromium (Cr), molybdenum (Mo), tungsten (W), silver (Ag), gold (Au), and any combination thereof.
5. The detection system of claim 1 , wherein the substrate comprises an organic material selected from a synthetic polymer, a biopolymer, a self-assembled monolayer (SAM), a bilayer membrane and any combination thereof.
6. The detection system of claim 1 , wherein the substrate comprises an organometallic complex.
7. The detection system of claim 1 , wherein the two or more analytes comprise a first analyte and a second analyte, and at least one of the plurality of sample segments has an affinity selective to the first analyte.
8. The detection system of claim 7 , wherein at least another of the plurality of sample segments has an affinity selective to the second analyte.
9. The detection system of claim 1 , wherein the optical interference layer comprises at least one metal oxide.
10. The detection system of claim 1 , wherein the optical interference layer has a refractive index of equal to or more than 2.
11. The detection system of claim 1 , wherein the plurality of sample segments is arranged in a two-dimensional array.
12. A method of detecting two or more analytes in a sample, the method comprising:
providing a sample suspected of containing two or more analytes to be detected;
contacting the sample with at least one sensor, the sensor comprising:
a substrate; and
an optical interference layer on the substrate, the optical interference layer comprising a plurality of sample segments, wherein two or more of the plurality of sample segments are configured to have different affinities to the two or more analytes wherein the optical interference layer comprises TiO 2 , ZrO 2 , HfO 2 , Cr 2 O 3 , or any combination thereof, and wherein the optical interference layer has a porous surface;
illuminating the two or more of the plurality of sample segments with at least one light source; and
detecting an interference spectrum from each of the two or more of the plurality of sample segments, thereby detecting the two or more analytes in the sample.
13. The method of claim 12 , further comprising comparing the interference spectrum from at least one of the plurality of sample segments with a reference interference spectrum.
14. The method of claim 13 , wherein the comparing step determines a concentration of at least one of the two or more analytes in the sample.
15. The method of claim 13 , wherein the comparing step identifies at least one of the two or more analytes in the sample.
16. The method of 13 , wherein the comparing step further comprises comparing changes in reflectance of the interference spectrum at one or more selected wavelengths.
17. The method of 13 , wherein the comparing step further comprises comparing changes in differential frequency of the interference spectrum at one or more selected wavelengths.
18. The method of claim 17 , wherein the one or more selected wavelengths are about 620 nm to about 740 nm.
19. The method of claim 12 , wherein at least one of the two or more analytes is a small molecule, a protein, a nucleic acid, an antibody, an antigen, a ligand, a DNA molecule, a RNA molecule, or any combination thereof.
20. The method of claim 12 , wherein detecting the interference spectrum comprises detecting the interference spectrum with at least one image detector.
21. The detection system of claim 1 , wherein each of the plurality of sample segments is surface treated with a surface treatment agent, and wherein the surface treatment agent is a carboxylic acid, a phosphoric acid, a bio-polymer, a synthetic polymer, or a metal organic compound.