Atypical hemolytic uremic syndrome (AHUS) biomarker proteins
View Patent ↗The disclosure provides biomarker proteins, a change in the concentration or activity level of which are associated with atypical hemolytic uremic syndrome (aHUS) or clinically meaningful treatment of aHUS with a complement inhibitor. Also provided are compositions and methods for interrogating the concentration and/or activity of one or more of the biomarker proteins in a biological fluid. The compositions and methods are useful for, among other things, evaluating risk for developing aHUS, diagnosing aHUS, determining whether a subject is experiencing the first acute presentation of aHUS, monitoring progression or abatement of aHUS, and/or monitoring response to treatment with a complement inhibitor or optimizing such treatment.
1. A method for treating a patient having atypical hemolytic uremic syndrome (aHUS) whose blood or urine has been determined to contain elevated levels at least two aHUS-associated biomarker proteins selected from the group consisting of TNFR1, MCP-1, IFN-γ, IL-6, a proteolytic fragment of complement component factor B, soluble C5b9 (sC5b9), prothrombin fragment F1+2, d-dimer, thrombomodulin, VCAM-1, von Willebrand Factor (vWF), complement component C5a, β2 microglobulin (β2M), clusterin, cystatin C, NAG, TIMP-1, NGAL, fatty acid binding protein 1 (FABP-1), albumin, CXCL9, KIM-1 and CCL5, soluble CD40 ligand (sCD40L), ICAM-1, IL-1 beta, IL-12 p70, IL-8, and vascular endothelial cell growth factor (VEGF), the method comprising administering to the patient an inhibitor of complement C5 in an amount and with a frequency sufficient to reduce the concentration of the at least two aHUS-associated biomarker proteins compared to the concentration measured in the patient's blood or urine prior to treatment with the complement C5 inhibitor.
2. The method of claim 1 , wherein the subject:
(a) has received dialysis at least once within the three months immediately prior to treatment with the complement C5 inhibitor; or
(b) is experiencing a first acute aHUS manifestation.
3. The method of claim 1 , wherein the reduced concentration of the at least two aHUS-associated biomarker proteins occurs within two weeks or two months after the first administration of the complement C5 inhibitor.
4. The method of claim 1 , wherein the subject is chronically treated with a complement C5 inhibitor.
5. The method of 1 , wherein the complement C5 inhibitor is selected from the group consisting of a small molecule, a polypeptide, a polypeptide analog, a peptidomimetic, and an aptamer.
6. The method of 1 , wherein the complement C5 inhibitor is selected from the group consisting of MB12/22, MB12/22-RGD, ARC187, ARC1905, SSL7, and OmCI.
7. The method of claim 1 , wherein the complement C5 inhibitor is an antibody, or an antigen-binding fragment thereof.
8. The method of claim 7 , wherein the antibody, or antigen-binding fragment thereof, is selected from the group consisting of a humanized antibody, a recombinant antibody, a diabody, a chimerized or chimeric antibody, a monoclonal antibody, a deimmunized antibody, a fully human antibody, a single chain antibody, an Fv fragment, an Fd fragment, an Fab fragment, an Fab′ fragment, and an F(ab′) 2 fragment.
9. The method of claim 7 , wherein the antibody, or antigen-binding fragment thereof, binds to complement component C5 and inhibits cleavage of C5 into fragments C5a and C5b.
10. The method of claim 9 , wherein the antibody is eculizumab or a variant of eculizumab.
11. The method of claim 10 , wherein the antigen-binding fragment is pexelizumab.
12. The method of claim 1 , wherein at least one of the aHUS-associated biomarkers is selected from the group consisting of: proteolytic fragment Ba of factor B, TNFR1, VCAM-1, D-dimer, thrombomodulin, and cystatin C.
13. The method of claim 1 , wherein the at least two aHUS-associated biomarker proteins are measured using an immunoassay, such as an enzyme-linked immunosorbent assay (ELISA) or a radioimmunoas say (RIA).
14. The method of claim 1 , wherein the biological fluid is blood, a blood fraction, or urine.
15. The method of claim 14 , wherein the blood fraction is serum or plasma.
16. The method of claim 1 , wherein:
(i) the concentration of at least one aHUS-associated biomarker is measured in two or more types of biological fluid; or
(ii) the concentration of a first of the at least two aHUS-associated biomarker proteins is measured in one type of biological fluid and the concentration of a second of the at least two aHUS biomarker proteins is measured in a second type of fluid.
17. The method of claim 1 , wherein:
(i) the concentration of CXCL9, IFN-γ, MCP-1, CCL5, sCD40L, or sTNFR1 in the serum of the subject is determined;
(ii) the concentration of at least one of (32 microglobulin (β2M), clusterin, cystatin C, NAG, TIMP-1, NGAL, fatty acid binding protein 1 (FABP-1), CXCL9, albumin, and KIM-1 in the urine of the subject is determined;
(iii) the concentration of NGAL, a proteolytic fragment of complement component factor B, soluble C5b9 (sC5b9), prothrombin fragment F1+2, D-dimer, thrombomodulin, or von Willebrand Factor (vWF) in the plasma of the subject is determined; and/or
(iv) the concentration of Ba in plasma obtained from the subject is determined.
18. The method of claim 1 , wherein the normal concentration of sTNFR1 is less than 2000 pg/mL.
19. The method of claim 1 , wherein the concentration of sTNFR1 in the biological sample is deemed elevated when it is:
(i) at least two fold greater than the normal concentration of sTNFR1; or
(ii) at least 10,000 μg/mL.