Polynucleotide molecules and uses thereof
View Patent ↗The present disclosure provides alternative sugar moieties and polynucleotides comprising such sugar moieties, and methods of use thereof.
1. An mRNA 30 to 5000 nucleotides in length encoding a polypeptide, the mRNA comprising at least one backbone moiety having the structure:
wherein B is a nucleobase;
or a salt thereof.
2. The mRNA of claim 1 , wherein B has the structure:
3. The mRNA of claim 1 , wherein B has the structure:
4. The mRNA of claim 1 , wherein B has the structure:
5. The mRNA of claim 1 , wherein B has the structure:
6. The mRNA of claim 1 , wherein B has the structure:
7. The mRNA of claim 1 , wherein B has the structure:
8. The mRNA of claim 1 , wherein B has the structure:
9. The mRNA of claim 1 , wherein B has the structure:
10. The mRNA of claim 1 , further comprising:
(a) a 5′ untranslated region;
(b) a 3′ untranslated region; and
(c) a 5′ cap structure.
11. The mRNA of claim 10 , wherein the 5′ cap structure has the structure:
or a salt thereof.
12. The mRNA of claim 10 , further comprising a poly-A tail 100 to 250 nucleotides in length at the 3′-terminus of the 3′-untranslated region.