METHOD OF PRODUCING RETINAL PIGMENT EPITHELIAL CELL
Provided are a production method of retinal pigment epithelial (RPE) cells that improves differentiation induction efficiency of pluripotent stem cells into RPE cells, and can provide highly pure RPE cells by a simple and easy operation in a short period, a culture method of RPE cells that can stably grow and culture a cell, a toxicity/efficacy evaluation method using RPE cells useful for transplantation therapy, and a therapeutic drug for a retinal disease. The invention relates to a production method of RPE cells, comprising adhesion culture of human pluripotent stem cells using a culture substrate coated with a laminin-E8 fragment, a culture method of RPE cells, comprising adhesion culture of RPE cells using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using RPE cells obtained by producing or culturing by the method, and a therapeutic drug for a retinal disease, containing the RPE cells.
1 . A production method of retinal pigment epithelial cells, comprising a step of adhesion culture of human pluripotent stem cells on a culture substrate coated with a laminin-E8 fragment.
2 . The method according to claim 1 , wherein the laminin-E8 fragment is laminin-511E8 fragment.
3 . The method according to claim 1 , wherein the step of adhesion culture is performed in the presence of a differentiation-inducing factor.
4 . The method according to claim 1 , wherein the human pluripotent stem cells are human iPS cells.
5 . A retinal pigment epithelial cell produced by the method according to claim 1 .
6 . A retinal pigment epithelial cell sheet produced by the method according to claim 1 .
7 . An amplification method of retinal pigment epithelial cells, comprising a step of adhesion culture of retinal pigment epithelial cells on a culture substrate coated with a laminin-E8 fragment.
8 . A purification method of retinal pigment epithelial cells, comprising a step of adhesion culture of retinal pigment epithelial cells on a culture substrate coated with a laminin-E8 fragment.
9 . A therapeutic drug for a retinal disease, comprising retinal pigment epithelial cells produced by the method according to claim 1 .
10 . A therapeutic drug for a retinal disease, comprising retinal pigment epithelial cells cultured by the method according to claim 7 .
11 . A method of treating a retinal disease in a human subject, comprising transplanting retinal pigment epithelial cells produced by the method according to claim 1 to the subject, thereby treating the retinal disease.
12 . A method of treating a retinal disease in a human subject, comprising transplanting retinal pigment epithelial cells cultured by the method according to claim 7 to the subject, thereby treating the retinal disease.
13 . A method of treating a retinal disease in a human subject, comprising transplanting a retinal pigment epithelial cell sheet produced by the method according to claim 1 to the subject, thereby treating the retinal disease.