Methods and compositions for induction of immune response
The invention provides a substantially antigen-free composition for induction of innate immune response in a bird or a mammal, the composition comprising a saponin, a sterol, a quaternary amine, and a polyacrylic polymer to the mammal or bird. Methods of using the composition are also provided.
1. A method of inducing an immune response to an infection caused by Gram-negative bacteria in a mammal or bird, comprising administering, prior to exposure of said mammal or bird to said Gram-negative bacteria, a substantially antigen-free composition comprising a saponin, a sterol, a quaternary amine, and a polyacrylic polymer to the mammal or bird.
2. The method of claim 1 , wherein said saponin is a triterpenoid saponin, said sterol is cholesterol, and said quaternary amine is DDA.
3. The method of claim 2 , wherein said triterpenoid saponin is Quil A or a purified fraction thereof.
4. The method of claim 1 , wherein the saponin is present in the amount of 1 μg to about 5,000 μg per dose, the sterol is present in the amount of 1 μg to about 5,000 μg per dose, the quaternary amine is present in the amount of 1 μg to about 5,000 μg per dose, and the polyacrylic polymer is present in the amount of 0.0001% volume to volume (v/v) to about 75% v/v.
5. The method of claim 1 , wherein the composition consists essentially of the saponin, the sterol, the quaternary amine, and the polyacrylic polymer.
6. The method of claim 1 , wherein said composition further comprises a glycolipid.
7. The method of claim 6 , wherein the glycolipid is N-(2-Deoxy-2-L-leucylamino-b-D-glucopyranosyl)-N-octadecyldodecanoylamide or a salt thereof.
8. The method of claim 6 , wherein said composition does not contain an immunostimulatory oligonucleotide.
9. The method of claim 6 , wherein the composition consists essentially of the glycolipid, the saponin, the sterol, the quaternary amine, and the polyacrylic polymer.
10. The method of claim 6 , wherein the glycolipid is present in the amount of 0.01 mg to about 10 mg per dose.
11. The method of claim 1 , wherein the composition further comprises an immunostimulatory oligonucleotide.
12. The method of claim 11 , wherein the composition consists essentially of the immunostimulatory oligonucleotide, the saponin, the sterol, the quaternary amine, and the polyacrylic polymer.
13. The method of claim 11 , wherein the composition consists essentially of the immunostimulatory oligonucleotide, the glycolipid, the saponin, the sterol, the quaternary amine, and the polyacrylic polymer.
14. The method of claim 11 , wherein the immunostimulatory oligonucleotide is present at the concentration of 20 μg to about 500 μg per ml.
15. The method of claim 1 , wherein said Gram-negative bacteria is Bordetella bronchiseptica.
16. The method of claim 1 , wherein said composition is antigen-free.