AH-7921 Detection
Antibodies, immunoassay methods and kits for the detection and determination of 3,4-dichloro-N-[(1-(dimethylamino)cyclohexyl)methyl]benzamide and 3,4-dichloro-N-[(1-(methylamino)cyclohexyl)methyl]benzamide, as well as the precursory immunogens, are described.
1. A polyclonal antibody raised against an immunogen of the structure:
wherein the antibody binds to (i) 3,4-dichloro-N-[(1-(dimethylamino)cyclohexyl)methyl]benzamide and (ii) 3,4-dichloro-N-[(1-(methylamino)cyclohexyl)methyl]benzamide, and
wherein the antibody has a relative cross-reactivity of 100% to 3,4-dichloro-N-[(1-(dimethylamino)cyclohexyl)methyl]benzamide and 94.186% to 3,4-dichloro-N-[(1-(methylamino)cyclohexyl)methyl]benzamide, wherein the cross-reactivity is measured in the presence of a detecting agent comprising N-(carboxypropyl)-3,4-dichloro-N-[1-(methylamino)cyclohexyl)methyl]benzamide conjugated to horseradish peroxidase.
2. The polyclonal antibody according to claim 1 , which has been purified by immunoglobulin precipitation, antigen-specific affinity purification, size-exclusion chromatography or ion exchange chromatography.
3. The polyclonal antibody according to claim 1 , wherein the antibody is adsorbed on or attached to a solid state device.
4. A method of detecting or determining 3,4-dichloro-N-[(1-(dimethylamino)cyclohexyl)methyl]benzamide or 3,4-dichloro-N-[(1-(methylamino)cyclohexyl)methyl]benzamide in a sample comprising:
i) contacting the sample with a detecting agent and the polyclonal antibody according to claim 1 ; and
ii) detecting or determining the amount of the detecting agent bound to the polyclonal antibody.
5. The method according to claim 4 , wherein the polyclonal antibody is adsorbed on or attached to a solid state device.
6. The method according to claim 4 , wherein the detecting agent is conjugated to a labelling agent, and wherein the labelling agent is horseradish peroxidase (HRP).
7. The method according to claim 6 , wherein the presence of the labelling agent is detected or determined by a colour change in response to reaction of the labelling agent with a substrate.
8. The method according to claim 7 , wherein the colour change is detected or determined by reading the absorbance at 450 nm.
9. The method according to claim 4 , wherein the presence of the detecting agent linked to the polyclonal antibody can be detected or determined in between about 2 hours and about 10 minutes.
10. A kit comprising the polyclonal antibody according to claim 1 .
11. The kit according to claim 10 , in which the polyclonal antibody is adsorbed on or attached to a solid state device.