Methods for sterilization and/or disinfection

1. A method for killing spores comprising:

contacting the spores with an aqueous solution comprising performic acid, wherein the performic acid is present in an amount of between about 1.0 weight percent and about 30 weight percent relative to total weight of the aqueous solution, wherein the contacting occurs at a temperature of between about 5° C. and about 35° C. for a period of time of between about 4 seconds and about 15 seconds, wherein the contacting effects at least a 4 log reduction in a number of spores capable of reproduction, metabolism, and/or growth, and wherein the method does not require activation by heat or ultraviolet light.

2. The process of claim 1 wherein the temperature is less than or equal to about 30° C. and the period of time is less than or equal to about 12 seconds.

3. The method of claim 1 wherein the temperature is less than or equal to about 27° C. and the period of time is less than or equal to about 10 seconds.

4. The method of claim 1 wherein the period of time is less than or equal to about 7 seconds.

5. The method of claim 1 wherein the period of time is less than or equal to about 5 seconds.

6. The method of claim 1 wherein the contacting effects at least a 5 log reduction in the number of spores capable of reproduction, metabolism, and/or growth.

7. The method of claim 1 wherein the contacting effects at least a 6 log reduction in the number of spores capable of reproduction, metabolism, and/or growth.

8. The method of claim 1 wherein the contacting effects at least a 7 log reduction in the number of spores capable of reproduction, metabolism, and/or growth.

9. The method of claim 1 wherein the aqueous solution is at least partially aerosolized.

10. The method of claim 1 further comprising aerosolizing at least a portion of the aqueous solution.

11. The method of claim 1 wherein the aqueous solution is aerosolized prior to the contacting.

12. The method of claim 1 further comprising generating the performic acid from a reaction between a peroxide and formic acid.

13. The method of claim 1 wherein the aqueous solution further comprises formic acid, hydrogen peroxide, or a combination thereof.

14. The method of claim 1 wherein the aqueous solution further comprises distilled water, deionized water, osmosis-purified water, tap water, or a combination thereof.

15. The method of claim 1 wherein the performic acid is present in an amount of at least about 1.5 weight percent based on total weight of the aqueous solution.

16. The method of claim 1 wherein the performic acid is present in an amount of at least about 2 weight percent based on total weight of the aqueous solution.

17. The method of claim 1 wherein the performic acid is present in an amount of at least about 3 weight percent based on total weight of the aqueous solution.

18. The method of claim 1 wherein the spores comprise bacterial spores.

19. The method of claim 1 wherein the spores comprise bacterial spores of a genus selected from the group consisting of Bacillus, Clostridium , and a combination thereof.

20. The method of claim 1 wherein the spores comprise bacterial spores selected from the group consisting of Geobacillus stearothermophilus, Bacillus atrophaeus, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans, Clostridium sporogenes, Clostridium botulinum, Bacillus subtilis globigii, Bacillus cereus, Bacillus circulans, Bacillus anthracis , and combinations thereof.

21. The method of claim 1 wherein the spores comprise Bacillus atrophaeus ATCC 9372 spores.

22. The method of claim 1 wherein the performic acid comprises an intramolecularly hydrogen-bonded monomeric form thereof.

23. The method of claim 1 wherein at least a portion of the spores are present on a surface.

24. The method of claim 1 wherein at least a portion of the spores are present on a surface of an article to be sterilized in an automated processing line.

25. The method of claim 1 wherein at least a portion of the spores are present on a surface, and wherein the method further comprises reducing residual oxidative activity at the surface.

26. The method of claim 25 wherein the reducing comprises treating the surface with a rinse solution comprising a chemical reagent configured to react with one or a plurality of residual oxidative species.

27. The method of claim 26 wherein the chemical reagent comprises an alkaline salt for shifting pH, an ionic metal, a pyruvate salt, an ascorbate salt, an enzyme, a bisulfite salt, a sulphite salt, a thiosulfate salt, activated carbon, clay, or a combination thereof.

28. The method of claim 26 wherein the chemical reagent comprises sodium hydroxide, catalyse, an iron salt, or a combination thereof.

29. A method for killing spores comprising:

contacting the spores with an aqueous solution comprising performic acid, wherein the performic acid is present in an amount of between about 1.0 weight percent and about 30 weight percent relative to a total weight of the aqueous solution, wherein the contacting occurs at a temperature of between about 5° C. and about 30° C. for a period of time of between about 4 seconds and about 10 seconds, wherein the contacting effects at least a 6 log reduction in a number of spores capable of reproduction, metabolism, and/or growth, and wherein the method does not require activation by heat or ultraviolet light.

30. The method of claim 29 further comprising aerosolizing at least a portion of the aqueous solution prior to the contacting.

31. The method of claim 29 further comprising generating the performic acid from a reaction between hydrogen peroxide and formic acid.

32. The method of claim 29 wherein the performic acid is present in an amount of at least about 1.5 weight percent based on total weight of the aqueous solution.

33. A method for killing spores comprising:

contacting the spores with an aqueous solution comprising performic acid, and/or a mixture of formic acid and hydrogen peroxide, in an amount of between about 1.0 weight percent and about 30 weight percent based on total weight of the aqueous solution, wherein at least a portion of the aqueous solution is in fluid communication with an aerosolized form thereof, wherein the contacting occurs at a temperature of between about 5° C. and about 30° C. for a period of time of between about 4 seconds and about 10 seconds, wherein the contacting effects at least a 6 log reduction in a number of spores capable of reproduction, metabolism, and/or growth, and wherein the method does not require activation by heat or ultraviolet light.

34. The method of claim 33 wherein the period of time is less than or equal to about 7 seconds.