Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer
View Patent ↗The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.
1. A transgenic plant comprising a Cry1Ab polynucleotide encoding a Cry1Ab insecticidal protein, and a DIG-3 polynucleotide encoding a DIG-3 insecticidal protein having at least 95% sequence identity with SEQ ID NO:2.
2. The transgenic plant of claim 1 , wherein said plant further comprises DNA encoding a third insecticidal protein selected from the group consisting of Cry1Fa, Cry1Be, and Cry2Aa.
3. The transgenic plant of claim 2 , said plant further comprising DNA encoding a fourth insecticidal protein selected from the group consisting of Cry1Be and Cry2Aa where the third insecticidal protein is Cry1Fa.
4. A seed of the plant of claim 1 , wherein said seed comprises the Cry1Ab polynucleotide encoding the Cry1Ab insecticidal protein and the DIG-3 polynucleotide encoding the DIG-3 insecticidal protein.
5. A plurality of plants comprising non-Bt refuge plants and the transgenic plants of claim 1 , wherein said refuge plants comprise less than 40% of said plurality of plants.
6. The plurality of plants of claim 5 , wherein said refuge plants comprise less than 30% of said plurality of plants.
7. The plurality of plants of claim 5 , wherein said refuge plants comprise less than 20% of said plurality of plants.
8. The plurality of plants of claim 5 , wherein said refuge plants comprise less than 10% of said plurality of plants.
9. The plurality of plants of claim 5 , wherein said refuge plants comprise less than 5% of said plurality of plants.
10. The plurality of plants of claim 5 , wherein said refuge plants are in blocks or strips.
11. A mixture of seeds comprising refuge seeds from non-Bt refuge plants and the seeds of claim 4 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.
12. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 30% of all the seeds in the mixture.
13. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 20% of all the seeds in the mixture.
14. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.
15. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.
16. A method of managing development of resistance to a Cry protein by an insect, said method comprising planting seeds to produce the plants of claim 5 .
17. The plurality of plants of claim 5 , wherein said plants occupy more than 10 acres.
18. The plant of claim 1 , wherein said plant is selected from the group consisting of corn, soybeans, and cotton.
19. The plant of claim 18 , wherein said plant is corn.
20. A non-totipotent plant cell comprising a Cry1Ab polynucleotide encoding a Cry1Ab insecticidal protein, and a DIG-3 polynucleotide encoding the DIG-3 insecticidal protein of SEQ ID NO:2.
21. A method of controlling a corn borer insect, wherein said method comprises contacting said insect or the environment of said insect with an effective amount of a composition that contains a Cry1Ab insecticidal protein and further contains the DIG-3 insecticidal protein of SEQ ID NO:2.
22. The method of claim 21 , wherein said composition is a plurality of plant cells.
23. A method of producing the composition of claim 22 , wherein said method comprises reproducing said cells.