Recombinant acetogenic bacterium for the conversion of methane to products
View Patent ↗The invention provides a recombinant, acetogenic bacterium that consumes a substrate comprising CH 4 and converts at least a portion of the CH 4 to a product. In particular, the bacterium of may comprise one or more of exogenous methane monooxygenase (MMO), exogenous nitrite reductase (NIR), and exogenous nitric oxide dismutase (NOD). The invention further provides a method for producing a product comprising providing a substrate comprising CH 4 to a culture comprising a recombinant, acetogenic bacterium, whereby the bacterium converts at least a portion of the CH 4 to a product.
1. A recombinant bacterium that consumes a substrate comprising CH 4 and converts at least a portion of the CH 4 to a product, wherein the bacterium comprises a Wood-Ljungdahl pathway and comprises exogenous Methylomirabilis oxyfera methane monooxygenase (MMO).
2. The bacterium of claim 1 , wherein the bacterium further comprises exogenous nitrite reductase (NIR) and/or exogenous nitric oxide dismutase (NOD).
3. The bacterium of claim 2 , wherein the nitrite reductase is Methylomirabilis oxyfera nitrite reductase and/or the nitric oxide dismutase is Methylomirabilis oxyfera nitric oxide dismutase.
4. The bacterium of claim 2 , wherein the bacterium further comprises exogenous methanol methyltransferase.
5. The bacterium of claim 1 , wherein the bacterium is a member of genus Clostridium or Acetobacterium.
6. The bacterium of claim 1 , wherein the bacterium is derived from Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei , or Acetobacterium woodii.
7. The bacterium of claim 1 , wherein the substrate further comprises one or more of CO, CO 2 , and H 2 .
8. The bacterium of claim 1 , wherein the substrate further comprises one or more of NO 2 − and NO 3 − .
9. The bacterium of claim 1 , wherein CH 4 is the sole carbon source for the bacterium.
10. The bacterium of claim 1 , wherein the product comprises one or more of ethanol, acetate, butanol, butyrate, 2,3-butanediol, lactate, butene, butadiene, methyl ethyl ketone (2-butanone), ethylene, acetone, isopropanol, lipids, 3-hydroxypropionate (3-HP), isoprene, fatty acids, 2-butanol, 1,2-propanediol, and 1-propanol.
11. A method for producing a product comprising providing a substrate comprising CH 4 to a culture comprising the bacterium of claim 1 , whereby the bacterium converts at least a portion of the CH 4 to a product.
12. The method of claim 11 , wherein the bacterium further comprises exogenous nitrite reductase (NIR) and/or exogenous nitric oxide dismutase (NOD).
13. The method of claim 12 , wherein the nitrite reductase is Methylomirabilis oxyfera nitrite reductase, and/or the nitric oxide dismutase is Methylomirabilis oxyfera nitric oxide dismutase.
14. The method of claim 12 , wherein the bacterium further comprises methanol methyltransferase.
15. The method of claim 11 , wherein the bacterium is a member of genus Clostridium or Acetobacterium.
16. The method of claim 11 , wherein the bacterium is derived from Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei , or Acetobacterium woodii.
17. The method of claim 11 , wherein the substrate further comprises one or more of CO, CO 2 , and H 2 .
18. The method of claim 11 , wherein the substrate further comprises one or more of NO 2 − and NO 3 − .
19. The method of claim 11 , wherein CH 4 is the sole carbon source for the bacterium.
20. The method of claim 11 , wherein the product comprises one or more of ethanol, acetate, butanol, butyrate, 2,3-butanediol, lactate, butene, butadiene, methyl ethyl ketone (2-butanone), ethylene, acetone, isopropanol, lipids, 3-hydroxypropionate (3-HP), isoprene, fatty acids, 2-butanol, 1,2-propanediol, and 1-propanol.