Self-contained biological assay apparatus, methods, and applications
View Patent ↗A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
1. An automated process, comprising:
providing a self-contained biological assay apparatus including a reagent dispensing system and a pneumatic manifold that operates a microfluidic system having a fluidic transport layer and a plurality of fluidic channels disposed in the fluidic transport layer, and a plurality of reservoirs attached to the fluidic transport layer:
introducing a fluid test sample containing a target nucleic acid into one of the plurality of reservoirs fluidically connected to one of the fluidic channels;
providing at least one liquid reagent to the fluidic channel from at least one reservoir that is in fluid connection with the fluidic transport layer;
combining the fluid test sample and the at least one reagent in the fluidic transport layer, one of the plurality of reservoirs or a temperature-controlled amplification/reaction reactor;
transporting the fluid test sample and the at least one reagent to the temperature-controlled amplification/reaction reactor that is in operative communication with the fluidic transport layer if not combined there;
incubating the fluid test sample and the at least one reagent in the temperature-controlled amplification/reaction reactor under conditions sufficient to permit the target nucleic acid sequence to be amplified;
transporting the fluid test sample and the at least one reagent to an analysis reservoir via the fluidic channel; and
analyzing the amplified target nucleic acid sequence from the fluid test sample, wherein the fluid test sample is automatically transported from a fluid sample introduction reservoir in the fluidic transport layer to the analysis reservoir separately from any other samples, all in a single microfluidic chip, via a fluidic channel and independent of contact with the pneumatic manifold and the reagent dispensing system.
2. The automated process of claim 1 , wherein the transporting and combining steps are accomplished by pumping the fluid test sample and at least one reagent through the fluidic transport layer via at least one multi-valve diaphragm pump that is operated by the pneumatic manifold.
3. The automated process of claim 1 , wherein the analyzing step further comprises performing a microarray analysis in a microarray analysis reservoir in the microfluidic system.
4. The automated process of claim 3 , further comprising:
providing a microarray analysis membrane in the microarray analysis reservoir;
flowing a fluid over a top surface of the microarray analysis membrane; and
removing the fluid substantially through a fluid exit route along a periphery of the microarray analysis membrane.
5. The automated process of claim 3 , further comprising:
providing a microarray analysis membrane in the microarray analysis reservoir; and,
flowing a fluid alternatively back and forth over a top surface of the microarray analysis membrane.
6. The automated process of claim 4 , further comprising providing heat to the microarray analysis membrane.
7. The automated process of claim 1 , wherein the analyzing step comprises detecting an interaction between the amplified target nucleic acid sequence and a probe for the target nucleic acid sequence.