Methods for cellobiosan utilization
View Patent ↗Disclosed herein are enzymes useful for the degradation of cellobiosan in materials such a pyrolysis oils. Methods of degrading cellobiosan using enzymes or organisms expressing the same are also disclosed.
1. A method for degrading cellobiosan, comprising contacting a cellobiosan containing material with a microbial β-glucosidase enzyme selected from Aspergillus niger β-glucosidase 1 (bgl1), Phanerochaete chrysosporium β-glucosidase 1A (bgl1A), Agrobacterium sp. β-glucosidase (abg) or Thermotoga maritima β-glucosidase (bglA).
2. The method of claim 1 , wherein the β-glucosidase enzyme is Aspergillus niger β-glucosidase 1 (bgl1) or Phanerochaete chrysosporium β-glucosidase 1A (bgl1A).
3. The method of claim 1 , wherein the cellobiosan containing material is a pyrolysis oil obtained from biomass.
4. The method of claim 1 , wherein the cellobiosan containing material is a fraction obtained by fractionating a pyrolysis oil.
5. The method of claim 4 , wherein the pyrolysis oil fraction is an aqueous fraction.
6. A method for producing levoglucosan and glucose from cellobiosan, comprising:
a) contacting the cellobiosan with at least one microbial β-glucosidase selected from Aspergillus niger β-glucosidase 1 (bgl1), Phanerochaete chrysosporium β-glucosidase 1A (bgl1A), Agrobacterium sp. β-glucosidase (abg) or Thermotoga maritima β-glucosidase (bglA),
b) isolating at least one of the levoglucosan or glucose products.
7. The method of claim 6 , wherein the β-glucosidase is Aspergillus niger β-glucosidase 1 (bgl1) or Phanerochaete chrysosporium β-glucosidase 1A (bgl1A).
8. The method of claim 6 , wherein the cellobiosan is contained within a pyrolysis oil obtained from biomass.
9. The method of claim 6 , wherein the cellobiosan is contained within a fraction obtained by fractionating a pyrolysis oil.
10. The method of claim 9 , wherein the pyrolysis oil fraction is an aqueous fraction.
11. The method of claim 7 , wherein the β-glucosidase is Aspergillus niger β-glucosidase 1 (bgl1).
12. The method of claim 7 , wherein the β-glucosidase is Phanerochaete chrysosporium β-glucosidase 1A (bgl1A).
13. The method of claim 6 , wherein the β-glucosidase is Agrobacterium sp. β-glucosidase (abg) or Thermotoga maritima β-glucosidase (bglA).
14. The method of claim 13 , wherein the β-glucosidase is Agrobacterium sp. β-glucosidase (abg).
15. The method of claim 13 , wherein the β-glucosidase is Thermotoga maritima β-glucosidase (bglA).
16. The method of claim 2 , wherein the β-glucosidase enzyme is Aspergillus niger β-glucosidase 1 (bgl1).
17. The method of claim 2 , wherein the β-glucosidase enzyme is Phanerochaete chrysosporium β-glucosidase 1A (bgl1A).
18. The method of claim 1 , wherein the β-glucosidase enzyme is Agrobacterium sp. β-glucosidase (abg) or Thermotoga maritima β-glucosidase (bglA).
19. The method of claim 18 , wherein the β-glucosidase enzyme is Agrobacterium sp. β-glucosidase (abg).
20. The method of claim 18 , wherein the β-glucosidase enzyme is Thermotoga maritima β-glucosidase (bglA).