MICROORGANISMS FOR FATTY ACID PRODUCTION USING ELONGASE AND DESATURASE ENZYMES
Recombinant microorganisms engineered for the production of polyunsaturated fatty acids (PUFAs) are provided. Also provided are biomass, microbial oils, and food products and ingredients produced by or comprising the microorganisms of the invention.
1 . A recombinant Labyrinthulomycetes cell producing a FAME profile comprising:
a. greater than 12% ARA; or
b. greater than 8% EPA; or
c. greater than 20% SA; or
d. greater than 10% OA; and
e. less than 10% DHA.
2 . The recombinant cell of claim 1 wherein the cell has a FAME profile having less than 5% DHA.
3 . The recombinant cell of claim 1 wherein the cell is viable on a medium that is not supplemented with a PUFA.
4 . The recombinant cell of claim 1 wherein the recombinant cell produces a FAME profile having greater than 12% ARA.
5 . The recombinant cell of claim 4 wherein the cell has a FAME profile having less than 5% DHA.
6 . The recombinant cell of claim 5 wherein the cell is viable on a medium that is not supplemented with a PUFA.
7 . The recombinant cell of claim 1 wherein the recombinant cell produces a FAME profile having greater than 8% EPA.
8 . The recombinant cell of claim 7 wherein the cell produces a FAME profile having less than 5% DHA.
9 . The recombinant cell of claim 8 wherein the cell is viable on a medium that is not supplemented with a PUFA.
10 . The recombinant cell of claim 1 wherein the recombinant cell produces a FAME profile having greater than 25% SA.
11 . The recombinant cell of claim 10 wherein the cell produces a FAME profile having less than 5% DHA.
12 . The recombinant cell of claim 11 wherein the cell is viable on a medium that is not supplemented with a PUFA.
13 . The recombinant cell of claim 1 wherein the recombinant cell produces a FAME profile having greater than 10% OA.
14 . The recombinant cell of claim 13 wherein the cell produces a FAME profile having less than 5% DHA.
15 . The recombinant cell of claim 14 wherein the cell is viable on a medium that is not supplemented with a PUFA.
16 . A biomass produced by a recombinant Labyrinthulomycetes cell of claim 1 and having a FAME profile comprising a parameter selected from the group consisting of: greater than 8% EPA, greater than 12% ARA, greater than 12% OA, greater than 15% PA, and wherein the parameter is produced by an exogenous pathway.
17 . The biomass of claim 16 wherein the biomass has a FAME profile of greater than 10% EPA.
18 . The biomass of claim 16 wherein the biomass has a FAME profile of greater than 12% ARA.
19 . The biomass of claim 17 wherein the biomass has a FAME profile having less than 10% DHA.
20 . A food product or ingredient comprising the biomass of claim 16 .
21 . A food product or ingredient comprising the biomass of claim 19 .
22 . A microbial oil comprising at least one polyunsaturated fatty acid synthesized by a Labyrinthulomycetes cell, wherein the oil has a FAME profile having a content of EPA that is higher than the content of DHA.
23 . The microbial oil of claim 22 wherein the cell is a member of a genus selected from the group consisting of: Aurantiochytrium , a Schizochytrium , a Thraustochytrium , and an Oblongichytrium.
24 . The microbial oil of claim 23 wherein the FAME profile is greater than 10% EPA.
25 . The microbial oil of claim 24 wherein the oil has a FAME profile of less than 5% DHA.
26 . The microbial oil of claim 23 wherein the oil has a FAME profile having greater than 10% EPA and less than 1% DHA.
27 . The microbial oil of claim 22 wherein the microbial oil is an extracted and unconcentrated oil.
28 . A microbial oil comprising at least one polyunsaturated fatty acid synthesized by a Labyrinthulomycetes cell, wherein the oil has a FAME profile having a content of ARA of greater than 15%.
29 . The microbial oil of claim 28 wherein the cell is a member of a genus selected from the group consisting of: Aurantiochytrium , a Schizochytrium , a Thraustochytrium , and an Oblongichytrium.
30 . The microbial oil of claim 29 having a FAME profile with less than 5% DHA.
31 . A food product or food ingredient comprising the microbial oil of claim 22 .
32 . The food product of claim 31 wherein the food product is animal feed.