Media mixing chamber
A mixing apparatus for reconstituting a powdered cell culture media. The apparatus includes at least one fluid chamber, an influent port at a lower portion of the fluid chamber. The apparatus further includes a geometric fluid flow aid positioned in the fluid chamber and an effluent port at the top of the fluid chamber. A powdered cell culture media is provided in the fluid to be mixed with a fluid provided by the influent port. The effluent port is configured to allow reconstituted media to exit the fluid chamber.
1. A mixing apparatus for reconstituting a powdered cell culture media, the apparatus comprising:
at least one fluid chamber;
a first cap enclosing a lower portion of the fluid chamber;
an influent port in the first cap;
a second cap enclosing an upper portion of the fluid chamber;
a geometric fluid flow aid coupled to the first cap and extending away from the lower cap in a direction towards a center of the fluid chamber, wherein the geometric fluid flow aid comprises a geometric shape having a decreasing diameter in the direction towards the center of the fluid chamber;
a filter material associated with the second cap, wherein the filter material is used to keep the powdered cell culture media inside the fluid chamber until dissolved;
an effluent port in the second cap;
wherein a powdered cell culture media is provided in the fluid to be mixed with a fluid provided by the influent port; and
wherein the effluent port is configured to allow reconstituted media to exit the fluid chamber.
2. The mixing apparatus of claim 1 , further comprising a second geometric fluid flow aid, wherein the first geometric fluid flow aid is positioned at a bottom of the fluid chamber and the second geometric fluid flow aid is positioned at the top of the fluid chamber.
3. The mixing apparatus of claim 2 , wherein the first and the second geometric fluid flow aids are substantially cone-shaped.
4. The mixing apparatus of claim 1 , further comprising a second fluid chamber positioned above the at least fluid chamber, wherein the first and the second fluid chambers are axially aligned.
5. The mixing apparatus of claim 4 , wherein the second fluid chamber is provided with an additive to be added to the reconstituted fluid once it exits the first chamber.
6. The mixing apparatus of claim 1 , further comprising a second influent flow port at a top portion of the fluid chamber.
7. The mixing apparatus of claim 6 , wherein the first and the second influent ports are positioned on the same vertical plane.
8. The mixing apparatus of claim 6 , wherein the first and the second influent ports are tangentially oriented relative to an inner wall of the fluid chamber.
9. The mixing apparatus of claim 1 , wherein the mixing apparatus is configured for single use.
10. The mixing apparatus of claim 1 , wherein the ratio of the cross-sectional area of the first influent port (in inches) to flow rate (liters/minute) is in the range of 0.0015 to 0.0040.
11. The mixing apparatus of claim 1 , wherein the filter material is a porous filter used to keep the powdered cell culture media inside the fluid chamber until dissolved.
12. The mixing apparatus of claim 1 , wherein the filter material is a porous polypropylene or polyethylene.
13. The mixing apparatus of claim 1 , wherein the filter material has a porosity in the range of 30 to 200 microns.
14. The mixing apparatus of claim 1 , further comprising a hydrophobic vent filter between the effluent port and a sterilizing filter configured to allow air that is entrained in dissolved medium to vent.
15. The mixing apparatus of claim 1 , further comprising a quick-connect devices at the influent port so that the quick-connect device prevents the egress of the powdered cell culture media powder from the cartridge during storage and shipment.