Bacterial ghosts as carrier and targeting vehicles
The invention concerns the use of bacterial ghosts as carrier and targeting vehicles for active substances.
1. A method for preparation of a bacterial ghost containing an active substance encapsulated therein, comprising:
(i) preparing a bacterial ghost comprising a receptor, wherein the receptor is a heterologously expressed polypeptide integrated into a cytoplasmic membrane of the bacterial ghost comprising the receptor, and
(ii) encapsulating the active substance in the bacterial ghost comprising the receptor prepared in step (i), wherein the active substance is present in the bacterial ghost in the immobilized form by interactions with the receptor.
2. The method of claim 1 , wherein the active substance is immobilized on the receptor by non-covalent interactions.
3. The method of claim 1 , wherein the active substance is immobilized on the receptor by non-covalent interactions that are not antibody-antigen binding.
4. The method of claim 1 , wherein the active substance is not an antibody.
5. The method of claim 1 , wherein the active substance is immobilized by non-covalent interactions between avidin or streptavidin and biotin or biotin analogs on the receptor located on an inner side of the cytoplasmic membrane of the bacterial ghost.
6. The method of claim 1 , wherein the heterologously expressed polypeptide is a fusion polypeptide.
7. The method of claim 1 , wherein the heterologously expressed polypeptide is a fusion polypeptide containing streptavidin or avidin.
8. The method of claim 1 , wherein the active substance is a fusion polypeptide.
9. The method of claim 1 , wherein the active substance is derivatized with receptor binding groups.
10. The method of claim 1 , wherein the active substance is a biotinylated active substance.
11. The method of claim 1 , wherein the active substance is biotinylated alkaline phosphatase.