Sunflower mutant allele NSMA
View Patent ↗The present invention relates to a novel mutant allele of sunflower ( Helianthus annuus L.) designated NSMA, which confers white seed color to sunflower plants. The present invention also relates to plants and seeds of the family or line carrying the NSMA allele. In addition, the present invention is also directed to transferring the NSMA allele to plants in the same species lacking the allele, and is useful for producing novel types and varieties of white-seeded sunflowers.
1. A sunflower plant having white seed color, wherein said white seed color is conferred by mutant allele NSMA.
2. A sunflower seed containing a mutant allele designated NSMA, wherein representative samples of seed containing said allele NSMA were deposited under ATCC Accession Nos. PTA-122987 and PTA-123220.
3. A sunflower plant, or a part thereof, produced by growing the seed of claim 2 .
4. A tissue culture produced from protoplasts or cells from the plant of claim 3 , wherein said cells or protoplasts are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell root, root tip, pistil, anther, ovule, flower, shoot, stem, seed, and petiole.
5. A sunflower plant regenerated from the tissue culture of claim 4 , wherein said plant has mutant allele NSMA, and wherein said mutant allele confers white seed color.
6. A method for producing a hybrid sunflower seed, said method comprising crossing two sunflower plants and harvesting the resultant sunflower seed, wherein at least one sunflower plant is the sunflower plant of claim 3 .
7. A hybrid sunflower seed produced by the method of claim 6 .
8. A sunflower plant, or a part thereof, produced by growing said hybrid sunflower seed of claim 7 , wherein said plant contains mutant allele NSMA, and wherein said mutant allele confers white seed color.
9. A method of producing a sunflower plant wherein said method comprises introducing a transgene into the plant of claim 3 .
10. A sunflower plant produced by the method of claim 9 .
11. A method for transferring mutant allele NSMA to a different genetic background, wherein the method comprises:
(a) obtaining the hybrid sunflower plant of claim 8 ;
(b) backcrossing said hybrid plant to a recipient parent plant not having mutant allele NSMA to produce backcross progeny plants;
(c) selecting for backcross progeny plants that contain mutant allele NSMA;
(d) backcrossing said selected backcross progeny plants to said recipient parent;
(e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that contain mutant allele NSMA; and
(f) harvesting the resulting seed.
12. A plant produced from the seed of claim 11 , wherein said plant contains mutant allele NSMA and has white colored seeds.