Root-preferred promoter from a
View Patent ↗This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to:
a) a polylinker sequence;
b) a non metallothionein-like heterologous coding sequence; or
c) a combination of a) and b);
wherein said promoter comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1, and said promoter has below ground tissue preferred expression.
2. The nucleic acid vector of claim 1 , wherein said promoter is 1,875 bp in length.
3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1.
4. The nucleic acid vector of any one of claims 1 - 3 , further comprising a sequence encoding a selectable maker.
5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.
6. The nucleic acid vector of claim 5 , wherein the heterologous coding sequence encodes a selectable marker, an insecticidal resistance protein, a small RNA molecule, a site specific nuclease protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a nutritional quality protein or a DNA binding protein.
7. The nucleic acid vector of any of claim 1 - 3 or 5 , further comprising a 3′ untranslated polynucleotide sequence comprising SEQ ID NO:3 or SEQ ID NO:7, wherein the 3′ untranslated sequence is operably linked to said polylinker or said heterologous coding sequence.
8. The nucleic acid vector of any of claims 1 - 3 or 5 , further comprising a 5′ untranslated polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:4, wherein the 5′ untranslated sequence is operably linked to said polylinker or said heterologous coding sequence.
9. The nucleic acid vector of any of claims 1 - 3 or 5 , further comprising an intron sequence.
10. A non- Panicum plant comprising a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1 operably linked to a transgene heterologous coding sequence, wherein said heterologous coding sequence has below ground tissue preferred expression.
11. The plant of claim 10 , wherein said plant is selected from the group consisting of maize, wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.
12. The plant of claim 11 , wherein said plant is Zea mays.
13. The plant of any one of claims 10 - 12 , wherein the heterologous coding sequence is inserted into the genome of said plant.
14. The plant of claim 10 , further comprising a 3′ untranslated sequence comprising SEQ ID NO:7 or SEQ ID NO:3, wherein the 3′ untranslated sequence is operably linked to said heterologous coding sequence.
15. The plant of claim 10 , wherein said promoter is 1,875 bp in length.