IP Library › Granted Patent US 9,879,327
Granted Patent B2
US 9,879,327 · App. 15/098,433 · Granted Jan 30, 2018

Brassica genomic assays

Inventors: Matthew Curtis Harmon (Elkton, MD); Nancy L Henderson (Landenberg, PA); Cathy Xiaoyan Zhong (Wilmington, DE)
Assignee: E. I. DU PONT DE NEMOURS AND COMPANY
C12Q1/6895C07K14/415C12Q2600/13C12Q2600/158C12Q2600/166
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Quick Facts
Patent No.
US 9,879,327
App. No.
15/098,433
Granted
Jan 30, 2018
Kind
B2
Abstract

Methods and compositions for detecting, identifying, and quantifying Brassica A genomic DNA are described. The methods are specific to the Brassica A genome and do not cross-react with other Brassica species, crops or weedy relatives that could contribute to contamination of a canola field.

Claims (2)

1. An oligonucleotide composition comprising a nucleotide sequence SEQ ID NO: 22, or a nucleotide sequence complementary to SEQ ID NO: 22, wherein the oligonucleotide is (i) 32 to about 500 nucleotides; (ii) discriminates the Brassica A genome from the Brassica B and C genomes; and (iii) comprises a fluorescent detectable label.

2. A detection kit comprising the oligonucleotide composition of claim 1 and further comprising one or more reaction components to perform a quantitative reaction.

Assignments (1)
NUNC PRO TUNC ASSIGNMENT Recorded Nov 29, 2022
From: E.I. DU PONT DE NEMOURS AND COMPANY
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 063141/0155 →
Continuity (4)
Continuation 13900587 · May 23, 2013
Provisional Application 61777108 · Mar 12, 2013
Provisional Application 61650623 · May 23, 2012
Related Publication 20160222470A1 · Aug 4, 2016