Somatic haploid human cell line
The invention provides for a somatic fully haploid, karyotypically stable human cell line, e.g. obtainable by targeted deletion of one or more disomic chromosomal regions of a somatic near-haploid human parental cell, a method of producing the same, as well as the use of the cell line for producing isogenic cell variants, comprising genomic mutations at different genomic target sites, and a library of such cell variants.
1. A somatic fully haploid, karyotypically stable human cell line, which is the HAP2 cell line deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under accession number DSM ACC3220.
2. The cell line of claim 1 , obtained by targeted deletion of one or more disomic chromosomal regions of a somatic near-haploid human parental cell.