Application of laminin to corneal endothelial cell culture

Inventors: Noriko Koizumi (Kyotanabe, JP); Naoki Okumura (Kyotanabe, JP); Shigeru Kinoshita (Kyoto, JP); Friedrich E. Kruse (Erlangen, DE); Ursula Schloetzer-Schrehardt (Erlangen, DE)

Assignees: KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION; ActualEyes Inc.; CorneaGen, Inc.

C12N5/0621 A61K35/30 C12N2533/52

View Patent ↗

Loading inventors, assignments & file history…

Monitor This Case

Get email alerts when status or documents change.

Order Certified Copies

Most orders are placed with the USPTO same day — all within 24 business hours.

Order via The Patent Place →

Pre-filled with this patent's details

Quick Facts

Patent No.: US 11,624,053
App. No.: 15/100,147
Granted: Apr 11, 2023
Kind: B2

External Links

USPTO Patent Center↗ Full Patent PDF↗

Abstract

The present invention provides a method of culturing corneal endothelial cells. More specifically, the present invention provides a composition for culturing or growing corneal endothelial cells, comprising at least one agent consisting of laminins and fragments thereof which express in corneal endothelial cells. Specifically, the present invention can comprise laminin 511 (alpha5 beta1 gamma1) and laminin 512 (alpha5 beta2 gamma 1). The present invention further provides a culture container for corneal endothelial cells, which is coated with the composition of the present invention. Furthermore, the present invention provides a method for culturing corneal endothelial cells comprising the step of using the composition or the container of the present invention to culture the corneal endothelial cells.

Claims (3)

1. A method for promoting growth of differentiated corneal endothelial cells comprising contacting the cells in a culture container with a composition comprising from 0.1 μg/cm 2 to less than 1.5 μg/cm 2 of laminin 511-E8 fragment.

2. The method of claim 1 , wherein the differentiated corneal endothelial cells are isolated from human cornea.

3. A method for promoting growth of differentiated corneal endothelial cells comprising contacting the cells in a culture container with a composition comprising 0.1-1 μg/cm 2 of laminin 511-E8 fragment.

Assignments (6)

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 18, 2024

From: CORNEAGEN LLC

To: AURION BIOTECH, INC.

Reel/Frame 066161/0048 →

CHANGE OF NAME Recorded Jan 18, 2024

From: CORNEAGEN INC.

To: CORNEAGEN LLC

Reel/Frame 066344/0915 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 21, 2020

From: THE DOSHISHA

To: ACTUALEYES INC.