Compositions and methods for selecting maize plants with resistance to anthracnose stalk rot
Compositions and methods useful in identifying and/or selecting maize plants that have anthracnose stalk rot resistance are provided herein. The resistance may be newly conferred or enhanced relative to a control plant. The methods use markers to identify, select and/or construct resistant plants. Maize plants generated by the methods also provided.
1. A method of introgressing a QTL allele associated with anthracnose stalk rot resistance into a maize plant said method comprising:
a. screening a population with at least one marker to determine if one or more maize plants from the population comprises a QTL allele associated with anthracnose stalk rot resistance, wherein the screening comprises a nucleic acid assay for the detection of a marker comprising a “C” at position 201 in SEQ ID NO:9, a “C” at position 201 in SEQ ID NO:10, an “A” at position 201 in SEQ ID NO:11, a “G” at position 201 in SEQ ID NO:12, or an “A” at position 201 in SEQ ID NO:13; and
b. selecting from said population at least one maize plant comprising the QTL allele; and
c. crossing the at least one maize plant to a second maize plant;
d. evaluating progeny plants for the at least one marker allele associated with anthracnose stalk rot resistance; and
e. selecting progeny plants possessing the QTL allele at least one marker allele associated with anthracnose stalk rot resistance.
2. A method of selecting a maize plant that displays resistance to anthracnose stalk rot, the method comprising:
a. obtaining a first maize plant that comprises within its genome a haplotype comprising:
i. a “C” at position 201 in SEQ ID NO:9,
ii. a “C” at position 201 in SEQ ID NO:10,
iii. an “A” at position 201 in SEQ ID NO:11,
iv. a “G” at position 201 in SEQ ID NO:12, and
v. an “A” at position 201 in SEQ ID NO:13; and
b. crossing said first maize plant to a second maize plant;
c. screening progeny plants for the haplotype in (a) wherein the screening comprises a nucleic acid assay for the detection of a marker comprising a “C” at position 201 in SEQ ID NO:9, a “C” at position 201 in SEQ ID NO:10, an “A” at position 201 in SEQ ID NO:11, a “G” at position 201 in SEQ ID NO:12, or an “A” at position 201 in SEQ ID NO:1; and
d. selecting progeny plants that possess the haplotype in (a).