Circular extractor
View Patent ↗The present invention provides methods and systems for eluting and an analyte from a solid phase. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a solid phase to a second vessel, such as a microtiter plate well. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.
1. An extractor for use with an apparatus for eluting an analyte from a solid phase wherein said extractor comprises a cylindrical body having a top surface, a bottom surface, a circumferential surface and a plurality of chambers wherein each of said plurality of chambers defines an opening on each of said top surface, said bottom surface and said circumferential surface, thereby defining a plurality of openings on each of said top surface, said bottom surface and said circumferential surface;
wherein the extractor further comprises a compression ring; and
wherein during use said solid phase being sandwiched between said compression ring and said circumferential surface, such that said compression ring is positioned in overlying registry with said plurality of openings of said circumferential surface.
2. The extractor as defined in claim 1 wherein said plurality of openings on said top surface are located the same distance from the circumferential surface of the cylindrical body as each other.
3. The extractor as defined in claim 1 wherein said plurality of openings on said bottom surface are located the same distance from the circumferential surface of the cylindrical body as each other.
4. The extractor as defined in claim 1 wherein each of said plurality of openings on said top surface is in direct overlying registry with the corresponding of said plurality of openings on said bottom surface.
5. The extractor as defined in claim 1 wherein said plurality of openings on said circumferential surface are located the same distance from the top surface of the cylindrical body as each other.
6. The extractor as defined in claim 1 wherein each of said plurality of chambers defines a T-shape extending between said top surface, said bottom surface and said circumferential surface.
7. The extractor as defined in claim 1 wherein said solid phase is an electrophoresis gel or blotting membrane.
8. The extractor as defined in claim 1 wherein said solid phase is a strip.
9. The extractor as defined in claim 8 wherein said strip is an immobilized pH gradient (IPG) strip.
10. The extractor as defined in claim 1 wherein said cylindrical body comprises a central opening extending from said top surface to said bottom surface.
11. An apparatus for eluting an analyte from a solid phase wherein the analyte has been separated along a separation direction on said solid phase wherein said apparatus comprises an extractor as defined in claim 1 and a plurality of liquid distribution channels in fluid communication with a plurality of chambers in said extractor wherein each of said plurality of liquid distribution channels defines an inlet proximal to one of said plurality of chambers and an outlet fluidly connected to said inlet by a passageway extending therebetween.
12. The apparatus as defined in claim 11 wherein each of said plurality of liquid distribution channels extends outwards from each of said plurality of chambers of said extractor.
13. The apparatus as defined in claim 12 wherein each of said plurality of liquid distribution channels extends in a straight line and is the same length as each other of said plurality of liquid distribution channels.
14. The apparatus as defined in claim 13 wherein said plurality of liquid distribution channels are defined by a stack of laminated foils.
15. The apparatus as defined in claim 14 wherein said stack of laminated foils is supported by a base.
16. The apparatus as defined in claim 15 wherein said outlet of each of said plurality of liquid distribution channels is fluidly connected to a collection chamber.
17. The apparatus as defined in claim 16 wherein said collection chamber is a microtitre plate well.
18. The apparatus as defined in claim 17 wherein said microtire plate well is provided as part of a bendable row of microtitre plate wells.
19. The apparatus as defined in claim 18 further comprising a rod head positioned in overlying registry with said top surface of said cylindrical body of said extractor wherein said rod head comprises a plurality of rods each of which fits into one of said plurality of chambers of said extractor via one of said plurality of openings on said top surface of said extractor and exerts pressure into said plurality of chambers to facilitate elution of said analyte.
20. A method for eluting an analyte from a solid phase wherein said analyte has been separated along a separation direction in said solid phase, wherein said method comprises:
(i) providing an extractor as defined in claim 1 ;
(ii) positioning said solid phase in overlying registry with said plurality of openings on said circumferential surface of said cylindrical body of said extractor;
(iii) positioning the compression ring in overlying registry with said solid phase in order that said solid phase is sandwiched between said circumferential surface of said cylindrical body of said extractor and said compression ring; and
(iv) eluting said analyte from said solid phase.
21. The method as defined in claim 20 which further comprises the exertion of pressure into said plurality of chambers by means of positioning a rod head into the said plurality of openings on said top surface of said cylindrical body of said extractor to facilitate elution of said analyte.
22. The method as defined in claim 21 wherein said eluting step of said method is followed by a further step (iv) of transferring said eluted analyte along a plurality of liquid distribution channels.
23. The method as defined in claim 22 wherein each of said plurality of liquid distribution channels is fluidly connected to a collection chamber and wherein said method further comprises transfer of said eluted analyte from said plurality of chambers via said plurality of liquid distribution channels into said collection chambers.