CAP260, CAP174 AND K0224 HIV-1 ENVELOPES, PEPTIDE AND COMPOSITIONS
The invention is directed to HIV-1 envelope proteins and peptides, and compositions comprising the same to increase the breadth of vaccine coverage of the V1 V2 env region of clade C HIV-1.
1 . A synthetic peptide of FIG. 2 (SEQ ID NOs: 8-10, 29-31), or Example 1 (SEQ ID NO: 64, 68, 72), or FIG. 13 , or a combination thereof.
2 . A recombinant CAP260, CAP174 or Ko224 HIV-1 Envelope protein of FIG. 6 (SEQ ID NOs: 55-60), or FIG. 12 , or a combination thereof.
3 . The recombinant protein of claim 2 , wherein the protein is expressed as a gp160, gp140 or gp120.
4 . A nucleic acid encoding any one of the proteins of claim 2 .
5 . A vector comprising the nucleic acid of claim 4 .
6 . The vector of claim 5 , wherein the vector is suitable for use in a vaccine formulation or recombinant protein expression.
7 . The vector of claim 6 , where the vector is a pox virus vector.
8 . A composition comprising the synthetic peptide of claim 1 or a combination thereof; the recombinant protein of claim 2 or a combination thereof; the nucleic acid of claim 4 or a combination thereof; or the vector of claim 5 or a combination thereof.
9 . The composition of claim 8 comprising a trivalent combination of the CAP260, CAP174 and Ko224 peptides of claim 1 .
10 . The composition of claim 8 comprising a trivalent combination of the CAP260, CAP174 and Ko224 proteins of claim 2 , or the nucleic acids of claim 4 .
11 . The composition of claim 10 wherein the protein is present as a monomer.
12 . The composition of claim 8 , wherein the composition is immunogenic.
13 . The composition of claim 8 , wherein the composition comprises an adjuvant.
14 . A method of inducing antibodies in a subject comprising administering to the subject any one of the compositions of claim 8 .
15 . The method of claim 14 , wherein the composition of claim 8 is administered as a boost in an immunization regimen following administration of ZM-96gp120 as a prime, and TV-1 and 1086C gp120 envelope proteins as a boost.
16 . The method of claim 15 , wherein the composition of claim 8 is administered as a multiple boost.
17 . The method of claim 14 , wherein the composition of claim 8 is administered as a prime in an immunization regimen along with administration of ZM-96gp120 as a prime, and TV-1 and 1086C gp120 envelope proteins as a boost.
18 . The composition of claim 8 , wherein the composition further comprises TV1 gp120 envelope and 1086C gp120 envelope.