Immunoassay method and immunoassay system
An immunoassay method in which, by using a sensor chip on which a plurality of capture regions which capture a material to be detected by a first capturing body are arranged separated from each other, the material to be detected captured by the first capturing body is individually detected, wherein the plurality of capture regions are formed by using a different type of first capturing body depending on the type of a material to be detected to be captured, the method having: a detection processing order determination step of determining a detection processing order between the capture regions based on information about a detection processing order between the plurality of the capture regions; and a detection processing step of performing a detection processing for each of the capture regions according to the detection processing order between the plurality of the capture regions, and an immunoassay system using the method.
1. An immunoassay method using a sensor chip on which a plurality of capture regions are arranged separately from each other, wherein the plurality of capture regions capture a material to be detected by a first capturing body, wherein the plurality of capture regions are formed by using a different type of first capturing body depending on the type of a material to be detected to be captured, the method comprising:
bringing a sample into contact with the sensor chip so that a material is captured by the first capturing body in the plurality of capture regions;
determining a detection processing order in which a detection processing is to be individually performed for each of the respective capture regions, wherein the detection processing order is determined based on information about a detection processing order of the plurality of the capture regions; and
individually performing the detection processing for each of the capture regions in the determined detection processing order, wherein the detection processing comprises individually irradiating each capture region and detecting fluorescence emitted from the irradiated capture region, in the determined detection processing order.
2. The immunoassay method according to claim 1 , wherein the information about a detection processing order is information stored in the sensor chip, or information stored in a device in which a detection processing is performed which is referred to corresponding to information stored in the sensor chip.
3. The immunoassay method according to claim 2 , wherein the information about a detection processing order is information about a dissociation constant of the material to be detected.
4. The immunoassay method according to claim 2 , wherein the information about a detection processing order is information about a type of the material to be detected.
5. The immunoassay method according to claim 2 , wherein the information about a detection processing order is information in which the detection processing order is determined in advance.
6. The immunoassay method according to claim 1 , wherein the information about a detection processing order is information about a dissociation constant of the material to be detected.
7. The immunoassay method according to claim 6 , wherein information about a dissociation constant of the material to be detected is a first dissociation constant between the material to be detected and the first capturing body or a second dissociation constant between the material to be detected and a second capturing body labeled with a fluorescent material.
8. The immunoassay method according to claim 6 , wherein the information about a detection processing order is information about a type of the material to be detected.
9. The immunoassay method according to claim 7 , wherein the information about a detection processing order is information about a type of the material to be detected.
10. The immunoassay method according to claim 6 , wherein the information about a detection processing order is information in which the detection processing order is determined in advance.
11. The immunoassay method according to claim 1 , wherein the information about a detection processing order is information about a type of the material to be detected.
12. The immunoassay method according to claim 1 , wherein the information about a detection processing order is information in which the detection processing order is determined in advance.
13. The immunoassay method according to claim 1 , wherein the information about a detection processing order is information representing a degree of necessity of detection accuracy.
14. The immunoassay method according to claim 1 , wherein the information about a detection processing order is ascending order of a predicted measurement value of a fluorescence signal.
15. The immunoassay method according to claim 1 , wherein, when information stored in the sensor chip is not present, a detection processing order is determined such that a total time for performing the detection processing for all of the plurality of capture regions is minimized.
16. The immunoassay method according to claim 1 , further comprising simultaneously performing capturing of the material to be detected by the first capturing body and performing fluorescent labeling of a material to be detected by a second capturing body in the plurality of capture regions.
17. The immunoassay method according to claim 1 , wherein the detection processing comprises detecting, in each of the plurality of capture regions, a fluorescence signal by a surface plasmon-field enhanced fluorescence spectroscopy.