Water soluble fluorescent or colored dyes and methods for their use

1. A compound having the following structure (I):

or a salt, stereoisomer or tautomer thereof, wherein:

R 1a and R 1b are each independently hydroxyl or alkoxy; and

R 2a , R 2b , R 2c , R 2d , R 2e , R 2f , R 2g , R 2h , R 2i , R 2j , R 2k , R 2l , R 2m , R 2n , R 2o , R 2p , R 2q , R 2r , R 2s and R 2t are each independently H, halo or -L 1 -R 3 ;

R 3 is, at each occurrence, independently an analyte molecule or alkyl substituted with one or more of hydroxyl, protected hydroxyl, amino, alkylamino, alkoxy, polyalkyether, hydroxylalkoxy, aminoalkoxy, hydroxylpolyalkyether, aminopolyalkyether, phosphate, thiophosphate, phospho, thiophospho, phosphoalkylether, Ophosphoalkylether, thiophosphoalkylether, Othiophosphoalkylether, phosphoramidite or activated phosphorous; or R 3 is a microparticle; and

L 1 is an optional linker moiety.

2. The compound of claim 1 , wherein the compound has the following structure:

3. The compound of claim 1 , wherein the compound has the following structure (If):

4. The compound of claim 1 , wherein R 1a or R 1b is hydroxyl.

5. The compound of claim 1 , wherein R 1a or R 1b , or both, is alkoxy.

6. The compound of claim 1 , wherein at least one of R 2a , R 2b , R 2c , R 2d , R 2e , R 2f , R 2g or R 2h is halo.

7. The compound of claim 6 , wherein R 2b is bromo.

8. The compound of claim 1 , wherein at least one of R 2a , R 2b , R 2c , R 2d , R 2e , R 2f , R 2g or R 2h is -L 1 -R 3 .

9. The compound of claim 8 , wherein R 2b is -L 1 -R 3 .

10. The compound of claim 1 , wherein R 3 is, at each occurrence, independently alkyl substituted with one or more of hydroxyl, protected hydroxyl, amino, alkylamino, alkoxy, polyalkyether, hydroxylalkoxy, aminoalkoxy, hydroxylpolyalkyether, aminopolyalkyether, phosphate, thiophosphate, phospho, thiophospho, phosphoalkylether, Ophosphoalkylether, thiophosphoalkylether, Othiophosphoalkylether, phosphoramidite or activated phosphorous.

11. The compound of claim 10 , wherein R 3 has, at each occurrence, independently one of the following structures:

wherein:

n 1 , n 2 and n 3 are each independently an integer from 1 to 6; and

x and y are each independently an integer from 1 to 10.

12. The compound of claim 1 , wherein R 3 is, at each occurrence, independently an analyte molecule.

13. The compound of claim 12 , wherein the analyte molecule is a nucleic acid, peptide, carbohydrate, lipid, enzyme, receptor, receptor ligand, antibody, glycoprotein, aptamer, antigen, prion, drug, vitamin or small molecule.

14. The compound of claim 1 , wherein L 1 comprises a phosphate bond to the analyte molecule.

15. The compound of claim 14 , wherein L 1 has the following structure:

wherein n 1 , n 2 and n 3 are each independently an integer from 1 to 6.

16. The compound of claim 1 , wherein the compound has one of the following structures:

wherein R 3 is an analyte molecule.

17. The compound of claim 1 , wherein the compound has the following structure (Ib):

18. The compound of claim 1 , wherein the compound has the following structure (Ic):

19. The compound of claim 1 , wherein the compound has the following structure (Id):

20. The compound of claim 1 , wherein the compound has the following structure (Ie):

21. The compound of claim 1 , wherein R 2a , R 2b , R 2c , R 2d , R 2e , R 2f , R 2g , R 2h , R 2i , R 2j , R 2k , R 2l , R 2m , R 2n , R 2o , R 2p , R 2q , R 2r , R 2s and R 2t are each H.

22. The compound of claim 1 , wherein R 2a , R 2b , R 2c , R 2d , R 2e , R 2f , R 2g , R 2h , R 2i , R 2j , R 2k , R 2l , R 2m , R 2n , R 2o , R 2p , R 2q , R 2r and R 2s are each independently H, halo or -L 1 -R 3 , and R 2t is H.