DETECTION, MONITORING AND TREATMENT OF ACUTE MYOCARDIAL INFARCTION
Detection, monitoring and treatment of acute myocardial infarction (AMI) using a hybrid immunoassay to both Lp-PLA2 and ApoA1. Described herein are assays and method of performing them useful for the detection of AMI. Also described are methods of monitoring a patient at risk for, or suffering from, AMI. Also described herein are methods and assays for treating patient having or at risk of suffering from AMI.
1 . A method of treating, detecting or monitoring acute myocardial infarction (AMI) comprising:
exposing a blood sample from a patient to a solid phase support onto which an Lp-PLA2-binding molecule is coupled;
incubating the blood sample with an ApoA1-binding molecule; and
detecting the level of ApoA1 from the solid phase.
2 . A method of treating, detecting or monitoring acute myocardial infarction (AMI) comprising:
exposing a blood sample from a patient to a solid phase support onto which an Lp-PLA2-binding molecule is coupled;
incubating the solid phase support with an ApoA1-binding molecule;
washing the solid phase support;
detecting the level of ApoA1 from the solid phase; and
treating the patient for AMI if the amount of ApoA1 detected is below a threshold.
3 . The method of claim 1 , further comprising treating the patient for AMI if the level of ApoA1 is below a threshold.
4 . The method of claim 1 , further comprising treating the patient for AMI if the level of ApoA1 detected is below 0.7 ng/ml.
5 . The method of claim 1 , further comprising washing the solid phase support.
6 . The method of claim 1 , wherein exposing the blood sample from the patient to the solid phase support comprising adding the sample to a solid phase support to which an antibody or an antibody fragment specific for Lp-PLA2 is coupled.
7 . The method of claim 1 , wherein incubating the blood sample with the ApoA1-binding molecule comprises incubating with an antibody or an antibody fragment that binds ApoA1.
8 . The method of claim 1 , wherein detecting the level of ApoA1 from the solid phase support comprises reacting a horseradish peroxidase (HRP) bound to an antibody against ApoA1 to detect a signal and quantifying the detected signal.
9 . A system for detecting or monitoring acute myocardial infarction (AMI), the system comprising:
a first component comprising an Lp-PLA2 binding molecule coupled to a solid-phase support;
a reagent comprising an ApoA1 binding molecule to which a detection moiety is coupled.
10 . The system of claim 9 , wherein the first component comprises a solid-phase support to which an antibody or antibody fragment that binds to Lp-PLA2 has been coupled.
11 . The system of claim 9 , wherein the reagent comprises a solution including an ApoA1 antibody or antibody fragment coupled to a detection moiety.
12 . The system of claim 9 , further comprising a set of standards configured to calibrate the level of ApoA1 detected by the detection moiety.
13 . A system for detecting or monitoring acute myocardial infarction (AMI), the system comprising:
a first component comprising an LpPLA2 binding molecule coupled to a solid-phase support;
a first wash buffer;
a reagent comprising an ApoA1 binding molecule to which a detection moiety is coupled; and
a detection buffer.
14 . The system of claim 13 , wherein the first component comprises a solid-phase support to which an antibody or antibody fragment that binds to Lp-PLA2 has been coupled.
15 . The system of claim 13 , wherein the reagent comprises a solution including an ApoA1 antibody or antibody fragment coupled to a detection moiety.
16 . The system of claim 13 , further comprising a set of standards configured to calibrate the level of ApoA1 detected by the detection moiety.
17 . The method of claim 2 , further comprising treating the patient for AMI if the level of ApoA1 detected is below 0.7 ng/ml.
18 . The method of claim 2 , wherein exposing the blood sample from the patient to the solid phase support comprising adding the sample to a solid phase support to which an antibody or an antibody fragment specific for Lp-PLA2 is coupled.
19 . The method of claim 2 , wherein incubating the blood sample with the ApoA1-binding molecule comprises incubating with an antibody or an antibody fragment that binds ApoA1.
20 . The method of claim 2 , wherein detecting the level of ApoA1 from the solid phase support comprises reacting a horseradish peroxidase (HRP) bound to an antibody against ApoA1 to detect a signal and quantifying the detected signal.