Plants with increased yield and method for producing said plants
The invention relates to a method for increasing the yield and biomass of a plant, by means of an increase in the expression of the L-aspartate oxidase in the plant. The method according to the invention allows an increase in the photosynthetic capacities of the plants as a result of an increase in the quantities of NAD and the derivatives thereof in said plants. The invention relates to the plants produced by such a method.
1. A method for improving at least one phenotypic trait of a plant, said method comprising introgression of a nucleic acid sequence into the plant, wherein the nucleic acid sequence comprises a sequence encoding L-aspartate oxidase and comprises an exogenous promoter functionally linked to the sequence encoding L-aspartate oxidase,
wherein the at least one phenotypic trait is one or more selected from the group consisting of biomass, yield, abiotic stress resistance, biotic stress resistance, germination rate and growth rate,
wherein the nucleic acid sequence is present in a genome,
wherein the resulting plant overexpresses L-aspartate oxidase, and
wherein the overexpression of L-aspartate oxidase leads to increased amounts of NAD and derivatives thereof,
wherein the sequence encoding L-aspartate oxidase comprises SEQ ID NO: 1.
2. The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the yield of the plant.
3. The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the biomass production of the plant.
4. The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the abiotic stress resistance and/or the biotic stress resistance of the plant.
5. The method according to claim 1 , wherein the plant is selected from the group consisting of wheat, barley, rice, maize, sorghum, sunflower, rapeseed, soybean, cotton, pea, common bean, cassava, mango, banana, potato, tomato, pepper, melon, zucchini, watermelon, lettuce, cabbage, eggplant, and poplar.
6. A method for producing a plant exhibiting at least one improved phenotypic trait, comprising detection of the presence of a genetic element, linked to overexpression of L-aspartate oxidase in a donor plant, and transfer of the genetic element, linked to overexpression of L-aspartate oxidase in the donor plant thus detected, from the donor plant to a recipient plant,
wherein the at least one phenotypic trait is one or more selected from the group consisting of biomass, yield, abiotic stress resistance, biotic stress resistance, germination rate and growth rate,
and wherein the genetic element comprises SEQ ID NO: 1.
7. The method according to claim 6 , wherein detection is carried out by measuring L-aspartate oxidase enzymatic activity in the donor plant.
8. The method according to claim 1 , wherein the method comprises:
a) Providing a plant having a given level of expression of L-aspartate oxidase,
b) Providing a plant having an increased level of expression of L-aspartate oxidase in relation to the plant provided in a),
c) Crossing the plant provided in a) with the plant provided in b),
d) Generating progeny resulting from the crossing c),
e) Selecting among the progeny at least one plant having a higher level of expression of L-aspartate oxidase than that of the plant provided in b).
9. The method according to claim 8 comprising an additional step of crossing the plant selected in e) with the plant provided in b) followed by an additional step of selecting among the progeny produced at least one plant having a higher level of expression of L-aspartate oxidase than that of the plant selected in e).
10. A method for improving at least one phenotypic trait of a plant, said method comprising introgression of a nucleic acid sequence into the plant, wherein the nucleic acid sequence comprises a sequence encoding L-aspartate oxidase and comprises an exogenous promoter functionally linked to the sequence encoding L-aspartate oxidase,
wherein the at least one phenotypic trait is one or more selected from the group consisting of biomass, yield, abiotic stress resistance, biotic stress resistance, germination rate and growth rate,
wherein the nucleic acid sequence is present in a genome,
wherein the resulting plant overexpresses L-aspartate oxidase, and
wherein the overexpression of L-aspartate oxidase leads to increased amounts of NAD and derivatives thereof,
wherein the sequence encoding L-aspartate oxidase comprises SEQ ID NO: 1.
11. The method according to claim 1 , wherein the genetic element comprises a sequence having at least 90% homology with SEQ ID NO:1.
12. The method according to claim 1 , wherein the genetic element comprises a sequence having at least 95% homology with SEQ ID NO:1.
13. The method according to claim 1 , wherein the genetic element comprises a sequence having at least 98% homology with SEQ ID NO:1.
14. The method according to claim 6 , wherein the genetic element comprises a sequence having at least 90% homology with SEQ ID NO:1.
15. The method according to claim 6 , wherein the genetic element comprises a sequence having at least 95% homology with SEQ ID NO:1.
16. The method according to claim 6 , wherein the genetic element comprises a sequence having at least 98% homology with SEQ ID NO:1.