Systems and methods for growing and harvesting cells
This disclosure is directed to methods and systems for growing and/or harvesting cells. The method can include seeding cells on a plurality of carriers. The method can also include incubating the carriers under conditions suitable for cell growth.
1. A system for growing and harvesting cells, comprising:
a. a bioreactor comprising a vessel, said vessel comprising a first compartment, said vessel further comprising a second compartment positionable below said first compartment, said second compartment defined between a first perforated structure and a second perforated structure disposed above the first perforated structure, said second perforated structure moveably coupled to the vessel;
b. a plurality of carriers;
c. a rod coupled to the second perforated structure, the rod extending upward from the second perforated structure and through a port in an upper plate of the vessel; and
d. a locking element that engages said upper plate and said rod and locks said second perforated structure in position in said vessel,
wherein:
said bioreactor is configured to seed cells in said first compartment, while said plurality of carriers are disposed in said second compartment;
each of said carriers comprises a body, said body comprising one or more two-dimensional (2D) surfaces extending inwardly from a periphery of the body towards an interior of the body; and
said bioreactor is configured to incubate said cells together with said carriers in said bioreactor under conditions compatible with growth and survival of said cells.
2. The system of claim 1 , wherein when said locking element engages said upper plate and said rod and locks said second perforated structure in position in said vessel, said second perforated structure packs said carriers in said second compartment and prevents movement of said carriers.
3. The system of claim 1 , further including an agitation device configured to agitate the carriers in the vessel.
4. The system of claim 1 , further comprising a rotating cylinder, having spokes extending radially therefrom, said spokes disposed within said vessel.
5. The system of claim 1 , wherein said system is also configured for harvesting said cells at the conclusion of said incubating step, by contacting said carriers with a releasing agent.
6. The system of claim 5 , wherein said harvesting further comprises the step of agitating said carriers within said vessel in the presence of said releasing agent.
7. The system of claim 1 , wherein said bioreactor further comprises a cell-lift impeller, and said cell-lift impeller is activated during said seeding step.
8. The system of claim 7 , wherein said system further comprises a pump that is configured for generating an upward flow of culture media present in the bioreactor vessel during said seeding step.
9. The system of claim 8 , wherein the cell-lift impeller generates a downward flow of culture media during said seeding step, wherein the downward flow of culture media is partially or completely offset by the upward flow of culture media.
10. The system of claim 1 , wherein said first perforated structure comprises a plurality of pores, wherein pores distal from an inner portion of said first perforated structure are greater in size than pores proximal to the inner portion of said first perforated structure.
11. The system of claim 10 , wherein said first perforated structure defines a lower wall of said second compartment.
12. The system of claim 1 , wherein said second perforated structure comprises pores having a size in range of 120 microns to 1800 microns in average diameter.
13. The system of claim 12 , wherein said second perforated structure defines an upper wall of said second compartment.
14. The system of claim 1 , wherein said system is configured for:
a. manipulating said first perforated structure such that said carriers pass into a third compartment of said vessel, wherein said third compartment is disposed adjacent to said first perforated structure; and
b. imparting a rotational force to said carriers in said third compartment.
15. The system of claim 14 , wherein said first perforated structure folds into a configuration that allows the carriers pass around said first perforated structure from said second compartment into said third compartment of said vessel.