Modified DNA polymerases for improved amplification
The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
1. A method of amplifying nucleic acids in a biological sample, which method comprises a step of contacting the sample with a modified Taq polymerase the method comprising using a modified Taq polymerase having an amino acid sequence that is at least 95% identical to that of SEQ ID NO: 47 and includes an amino acid substitution relative to the Taq polymerase of SEQ ID NO: 38 at each of the following residues: A61, K346, S357, E507, 1707 and F749.
2. The method of claim 1 , wherein the modified Taq polymerase consists of the sequence of SEQ ID NO: 47.
3. In a method of amplifying nucleic acids in a biological sample, the improvement comprising contacting a sample with a modified Taq polymerase having an amino acid sequence that is at least 95% identical to that of SEQ ID NO: 47 and includes an amino acid substitution relative to the Taq polymerase of SEQ ID NO: 38, at each of the following residues: A61, K346, S357, E507, I707 and F749, and wherein the modified Taq polymerase has improved resistance to one or more inhibitors of nucleic acid amplification relative to the Taq polymerase having an amino acid sequence of SEQ ID NO: 38.