Proteins with repetitive bacterial-IG-like (BIG) domains present in
View Patent ↗The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.
1. A recombinant expression vector comprising a heterologous polynucleotide molecule encoding a polypeptide comprising an amino acid sequence greater than 90% identical to the amino acid sequence set forth as SEQ ID NO: 10.
2. The vector of claim 1 , wherein the heterologous polynucleotide is linked to a promoter.
3. The vector of claim 1 , wherein the polynucleotide molecule is from a Leptospira species.
4. The vector of claim 1 , wherein the encoded polypeptide comprises the amino acid sequence set forth as SEQ ID NO: 10.
5. The vector of claim 4 , wherein the encoded polypeptide consists of the amino acid sequence set forth as SEQ ID NO: 10.
6. The vector of claim 1 , wherein the polynucleotide comprises a nucleic acid sequence greater than 90% identical to the nucleic acid sequence set forth as SEQ ID NO: 9.
7. The vector of claim 6 , wherein the polynucleotide comprises the nucleic acid sequence set forth as SEQ ID NO: 9.
8. The vector of claim 7 , wherein the polynucleotide consists of the nucleic acid sequence set forth as SEQ ID NO: 9.
9. A host cell comprising the vector of claim 1 .
10. A method of producing a recombinant polypeptide, comprising purifying the recombinant polypeptide from the host cell of claim 9 .
11. A method of producing a recombinant polypeptide, comprising:
transforming a cell with the recombinant vector of claim 2 ;
expressing the polypeptide from the vector in the transformed cell; and
purifying the recombinant polypeptide.