Methods of producing antibodies to neonatal Fc receptor (FcRn)
The disclosure relates to antibodies that bind FcRn and methods of using these antibodies.
1. A method of producing an antibody specific to a neonatal Fc receptor (FcRn), the method comprising:
(i) culturing, under conditions suitable for expression of the antibody, a host cell comprising a first nucleic acid encoding a light chain variable region (V L ) of the antibody specific to the FcRn, and a second nucleic acid encoding a heavy chain variable region (V H ) of the antibody specific to the FcRn, wherein each of the first nucleic acid and the second nucleic acid is in operable linkage to a promoter; and
(ii) collecting the antibody specific to the FcRn produced in (i);
wherein:
the V L comprises a V L CDR1 that comprises the amino acid sequence of TGTGSDVGSYNLVS (SEQ ID NO:14); a V L CDR2 that comprises the amino acid sequence of GDSQRPS (SEQ ID NO:15); and a V L CDR3 that comprises the amino acid sequence of SSYAGSGIYV (SEQ ID NO:12) or ASYAGSGIYV (SEQ ID NO:13); and
the V H comprises a V H CDR1 that comprises the amino acid sequence of EYAMG (SEQ ID NO:22); a V H CDR2 that comprises the amino acid sequence of SIGSSGGQTKYADSVKG (SEQ ID NO:23); and a V H CDR3 that comprises the amino acid sequence of LAIGDSY (SEQ ID NO:24).
2. The method of claim 1 , wherein the V L comprises the amino acid sequence of SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:11.
3. The method of claim 1 , wherein the V H comprises the amino acid sequence of SEQ ID NO:9.
4. The method of claim 2 , wherein the V H comprises the amino acid sequence of SEQ ID NO:9.
5. The method of claim 1 , wherein the second nucleic acid further encodes a heavy chain constant region (C H ), which has a deletion corresponding to the C-terminal lysine residue at the last position of SEQ ID NO:17.
6. The method of claim 1 , wherein the first nucleic acid and the second nucleic are on the same expression vector.
7. The method of claim 1 , wherein the first nucleic acid and the second nucleic are on two different expression vectors.
8. The method of claim 1 , wherein the host cell is a bacterial cell, a yeast cell, an insect cell, a plant cell, or a mammalian cell.
9. The method of claim 8 , wherein the host cell is a mammalian cell.
10. The method of claim 9 , wherein the mammalian cell is selected from the group consisting of a Chinese Hamster Ovary (CHO) cell, a NS0 myeloma cell, a SP2 cell, a COS cell, and a mammary epithelial cell.
11. The method of claim 9 , wherein the host cell lacks an endogenous dihydrofolate reductase (dhfr − ).
12. The method of claim 11 , wherein the first nucleic acid or second nucleic acid further encodes a DHFR protein.