MULTI-COMPONENT ELECTROSPUN FIBER SCAFFOLDS
A scaffold may comprise a first polymeric electrospun fiber comprising a first material having a first degradation rate, and a second polymeric electrospun fiber comprising a second material having a second degradation rate different from the first degradation rate. The first degradation rate may substantially correspond to a cell infiltration rate, and the second degradation rate may be slower than the first degradation rate. Such a scaffold may be manufactured by electrospinning a first polymer fiber having a first degradation rate by ejecting a first polymer solution from a first polymer injection system onto a mandrel, and electrospinning a second polymer fiber having a second degradation rate different from the first degradation rate by ejecting a second polymer solution from a second polymer injection system onto a mandrel. Wound healing may be improved by applying such a scaffold to a portion of a wound.
1 . A scaffold comprising:
a first polymeric electrospun fiber comprising a first material having a first degradation rate; and
a second polymeric electrospun fiber comprising a second material having a second degradation rate different from the first degradation rate.
2 . The scaffold of claim 1 , wherein the first material and the second material are independently selected from the group consisting of polycaprolactone, chitosan, polydioxanone, polyglycolide, poly (lactide-co-caprolactone), poly (lactide-co-glycolide), poly-L-lactide, and combinations thereof.
3 . The scaffold of claim 1 , wherein the first material is polyglycolide and the second material is poly (lactide-co-caprolactone).
4 . The scaffold of claim 1 , wherein the first material is polydioxanone and the second material is poly (lactide-co-caprolactone).
5 . The scaffold of claim 1 , wherein the first degradation rate is from about 1 day to about 1 month.
6 . The scaffold of claim 1 , wherein the second degradation rate is from about 1 month to about 24 months.
7 . The scaffold of claim 1 , wherein the first degradation rate is from about 2 times to about 52 times higher than the second degradation rate.
8 . The scaffold of claim 1 , further comprising at least one biological cell selected from the group consisting of a differentiated cell, a multipotent stem cell, a pluripotent stem cell, a totipotent stem cell, an autologous cell, a syngeneic cell, an allogeneic cell, a bone marrow-derived stem cell, a cord blood stem cell, a mesenchymal cell, an embryonic stem cell, an induced pluripotent stem cell, an epithelial cell, an endothelial cell, a hematopoietic cell, an immunological cell, and any combination thereof.
9 . The scaffold of claim 1 , wherein the first polymeric electrospun fiber and the second polymeric electrospun fiber are present in a weight ratio selected from the group consisting of 1:1, 2:1, 3:1, 1:2, and 1:3.
10 . The scaffold of claim 1 , having a shape of a vascular graft, the vascular graft having a compliance, a burst pressure, and a suture retention strength.
11 . The scaffold of claim 8 , wherein the compliance is from about 2%/mmHg to about 14%/mmHg.
12 . The scaffold of claim 8 , wherein the burst pressure is from about 0.01 MPa to about 10 MPa.
13 . The scaffold of claim 8 , wherein the suture retention strength is from about 100 g to about 3500 g.
14 . The scaffold of claim 8 , wherein the vascular graft has a first end and a second end, and wherein the compliance varies from the first end to the second end.
15 . A scaffold comprising:
a first polymeric electrospun fiber comprising a first material having a first degradation rate that substantially corresponds to a cell infiltration rate; and
a second polymeric electrospun fiber comprising a second material having a second degradation rate slower than the first degradation rate.
16 . The scaffold of claim 15 , wherein the first material is polyglycolide and the second material is poly (lactide-co-caprolactone).
17 . The scaffold of claim 15 , wherein the first material is polydioxanone and the second material is poly (lactide-co-caprolactone).
18 . A kit comprising:
a scaffold comprising a first polymeric electrospun fiber comprising a first material having a first degradation rate, and a second polymeric electrospun fiber comprising a second material having a second degradation rate different from the first degradation rate;
and a sealable pouch.
19 . The kit of claim 18 , wherein the sealable pouch comprises at least one flashspun high-density polyethylene fiber, a foil component, and a desiccant component.
20 . The kit of claim 18 , wherein the scaffold is stable in ambient conditions for about two years.
21 . A method of manufacturing a scaffold, the method comprising:
electrospinning a first polymer fiber having a first degradation rate by ejecting a first polymer solution from a first polymer injection system onto a mandrel; and
electrospinning a second polymer fiber having a second degradation rate different from the first degradation rate by ejecting a second polymer solution from a second polymer injection system onto the mandrel.
22 . The method of claim 21 , wherein electrospinning the first polymer fiber and electrospinning the second polymer fiber are performed simultaneously.
23 . The method of claim 21 , wherein the first polymer solution and the second polymer solution each comprise a polymer independently selected from the group consisting of polycaprolactone, chitosan, polydioxanone, polyglycolide, poly (lactide-co-caprolactone), poly (lactide-co-glycolide), poly-L-lactide, and combinations thereof.
24 . The method of claim 21 , wherein the first polymer solution and the second polymer solution each comprise a solvent independently selected from the group consisting of acetone, dimethylformamide, dimethylsulfoxide, N-methylpyrrolidone, N,N-dimethylformamide, Nacetonitrile, hexanes, ether, dioxane, ethyl acetate, pyridine, toluene, xylene, tetrahydrofuran, trifluoroacetic acid, hexafluoroisopropanol, acetic acid, dimethylacetamide, chloroform, dichloromethane, water, alcohols, ionic compounds, and combinations thereof.
25 . A method of improving wound healing, comprising:
applying to a portion of a wound a scaffold comprising a first polymeric electrospun fiber comprising a first material having a first degradation rate, and a second polymeric electrospun fiber comprising a second material having a second degradation rate different from the first degradation rate.
26 . The method of claim 25 , further comprising preseeding the scaffold with at least one biological cell selected from the group consisting of a differentiated cell, a multipotent stem cell, a pluripotent stem cell, a totipotent stem cell, an autologous cell, a syngeneic cell, an allogeneic cell, a bone marrow-derived stem cell, a cord blood stem cell, a mesenchymal cell, an embryonic stem cell, an induced pluripotent stem cell, an epithelial cell, an endothelial cell, a hematopoietic cell, an immunological cell, and any combination thereof.
27 . The method of claim 25 , further comprising soaking the scaffold in a treatment selected from the group consisting of platelet-rich plasma, bone marrow, stromal vascular fraction, and combinations thereof.