Granted Patent
B2
US 9,944,916 · App. 15/148,056 · Granted Apr 17, 2018
Penicillin-G acylases
Inventors:
Goutami Banerjee (Hayward, CA); Jie Yang (Foster City, CA); Xiyun Zhang (Fremont, CA); Erika M. Milczek (New York, NY); Melissa Ann Mayo (Andover, MA); Stephan Jenne (Foster City, CA)
Assignee:
Codexis, Inc.
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Abstract
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Claims (2)
1. An engineered non-naturally occurring penicillin G acylase capable of removing the A1/B1/B29 tri-phenyl acetate protecting groups from insulin to produce free insulin, wherein said penicillin G acylase comprises SEQ ID NO:12.
2. The engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase is encoded by the polynucleotide sequence set forth in SEQ ID NO: 11.
Assignments (4)
SECURITY INTEREST
Recorded Feb 15, 2024
From: CODEXIS, INC.
To: INNOVATUS LIFE SCIENCES LENDING FUND I, LP, AS COLLATERAL AGENT
Reel/Frame 066600/0650 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 31, 2016
From: MERCK SHARP & DOHME CORP.
To: CODEXIS, INC.
Reel/Frame 040178/0040 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 5, 2016
From: MILCZEK, ERIKA M.
To: MERCK SHARP & DOHME CORP.
Reel/Frame 039353/0047 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 3, 2016
From: BANERJEE, GOUTAMI; YANG, JIE; ZHANG, XIYUN; MAYO, MELISSA ANN; JENNE, STEPHAN
To: CODEXIS, INC.
Reel/Frame 038797/0006 →
Continuity (2)
Provisional Application
62158118
· May 7, 2015