Class of glyphosate resistance genes
The present disclosure relates to a novel class of EPSPS enzymes, and nucleic acids useful in encoding the same.
1. A method for producing a glyphosate-tolerant plant material, the method comprising:
transforming a plant material with a nucleic acid molecule comprising a promoter operably linked to a polynucleotide encoding a Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant, wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant comprises a polypeptide that is at least 95% identical SEQ ID NO: 1.
2. The method according to claim 1 , wherein the transformed plant material further comprises a heterologous a-ketoglutarate-dependent dioxygenase-1 (AAD-1) or a heterologous α-ketoglutarate-dependent dioxygenase-12 (AAD-12).
3. The method according to claim 1 , wherein the plant material is a plant cell, callus, plant tissue, plant organ, or plant part.
4. The method according to claim 3 , further comprising regenerating a transgenic plant from the plant material.
5. The method according to claim 1 , wherein the plant material is a whole plant.
6. The method according to claim 1 , wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant further comprises a chloroplast transit peptide (CTP).
7. The method according to claim 6 , wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant is at least 95% identical to a protein selected from the group consisting of SEQ ID NOs:185-190.
8. A nucleic acid molecule comprising a polynucleotide operably linked to a heterologous promoter that is functional in a plant cell, the polynucleotide encoding a Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant, wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant comprises a polypeptide that is at least 95% identical SEQ ID NO: 1.
9. The nucleic acid molecule of claim 8 , wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant further comprises a chloroplast transit peptide (CTP).
10. The nucleic acid molecule of claim 9 , wherein the Streptomyces Class IV EPSPS means for providing glyphosate-tolerance to a plant is at least 95% identical to a protein selected from the group consisting of SEQ ID NOs: 185-190.
11. A transgenic, glyphosate-tolerant plant material comprising the nucleic acid molecule of claim 8 .
12. The transgenic, glyphosate-tolerant plant material of claim 11 , wherein the plant material is a plant cell, callus, plant tissue, plant organ, or plant part.
13. The transgenic, glyphosate-tolerant plant material of claim 11 , wherein the plant material further comprises a heterologous a-ketoglutarate-dependent dioxygenase-1 (AAD-1) or a heterologous a-ketoglutarate-dependent dioxygenase-12 (AAD-12).
14. A transgenic, glyphosate-tolerant plant comprising the nucleic acid molecule of claim 8 , wherein the heterologous promoter is functional in the plant.