Processed adipose tissue
The present disclosure provides tissue products produced from adipose-containing tissues, as well as methods for producing such tissue products. The tissue products can include decellularized and partially de-fatted tissues. In addition, the present disclosure provides systems and methods for using such products.
1. A method of producing a tissue product, comprising:
providing a sheet of tissue comprising adipose-containing transitional dermis;
treating the sheet of tissue to remove substantially all cellular material from the tissue; and
processing the sheet of tissue to partially remove lipid components such that the tissue product retains at least about 35% lipid content as a percentage of the overall tissue product by mass following processing.
2. The method of claim 1 , wherein the sheet of tissue comprises about 40-90% lipid content as a percentage of the overall tissue product by mass prior to processing.
3. The method of claim 1 , wherein substantially all cellular material is removed using one or more detergents.
4. The method of claim 3 , wherein the one or more detergents comprise at least a polyethylene glycol, Tris[2-(dimethylamino)ethyl]amine, sodium dodecyl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan mono-oleate.
5. The method of claim 1 , wherein processing the sheet of tissue to partially remove lipid components comprises exposing the sheet of tissue to an elevated temperature of about 40-50° C., to ultrasonic energy, or a combination of the elevated temperature and the ultrasonic energy.
6. The method of claim 1 , wherein the tissue product comprises at least about 3% extracellular matrix components as a percentage of the overall tissue product by mass following processing.
7. The method of claim 1 , further comprising exposing the tissue product to E-beam radiation.
8. The method of claim 1 , wherein processing the sheet of tissue to partially remove lipid components includes processing the sheet of tissue such that the tissue product contains between 35% and 50% lipid content as a percentage of the overall tissue product by mass.
9. A tissue product, comprising:
a sheet of decellularized adipose-containing transitional dermal matrix,
wherein a portion of lipid components have been removed from the sheet of decellularized adipose-containing transitional dermal matrix such that the tissue product retains at least about 35% lipid content as a percentage of the overall tissue product by mass.
10. The tissue product of claim 9 , wherein the tissue product has at most about 70% lipid content as a percentage of the overall tissue product by mass.
11. The tissue product of claim 9 , wherein the tissue product has at least about 3% extracellular matrix components as a percentage of the overall tissue product by mass.
12. A method of treatment comprising:
selecting a tissue site in a patient; and
implanting the tissue product of claim 9 on or into the tissue site.
13. The method of claim 12 , wherein the tissue site comprises adipose tissue or soft tissue.
14. The method of claim 12 , wherein the tissue site comprises a breast.
15. The method of claim 14 , wherein the tissue product is implanted to augment a tissue site.
16. The method of claim 12 , wherein the tissue site is a breast, a face, a buttock, an abdomen, a hip, or a thigh.