Methylation of the EPB41L3 gene or the promoter of the EPB41L3 gene in a test sample comprising cervical cells
The present invention relates to methods and kits for identifying, diagnosing, prognosing, and monitoring cervical cancer. These methods include determining the methylation status or the expression levels of particular genes, or a combination thereof.
1. A method comprising:
a) treating DNA isolated from cervical cells with a chemical reagent that selectively modifies a non-methylated cytosine residue relative to a methylated cytosine residue; and
b) detecting the treated DNA, wherein detecting the treated DNA comprises:
(i) amplifying the treated DNA with a pair of primers to form an amplification product; and
(ii) detecting the amplification product;
wherein:
(i) the primers comprise the nucleotide sequence of SEQ ID NO: 45 or SEQ ID NO:177; or
(ii) the amplification product comprises primer binding sites for primers comprising the nucleotide sequence of SEQ ID NO: 45 or SEQ ID NO:177.
2. The method of claim 1 , wherein the product is detected by a method selected from the group consisting of electrophoresis, hybridization, amplification, sequencing, ligase chain reaction, chromatography, mass spectrometry, and combinations thereof.
3. The method of claim 1 , wherein the chemical reagent comprises bisulfite ions.
4. The method of claim 3 , wherein the method further comprises contacting the DNA in the test sample with alkali after contacting the DNA in the test sample with bisulfite ions.
5. The method of claim 1 , wherein the test sample comprises squamous cells, nucleic acids from squamous cells, adenocarcinoma cells, nucleic acids from adenocarcinoma cells, adenosquamous cell carcinoma cells, nucleic acids from adenosquamous carcinoma cells, or any combination thereof.
6. The method of claim 1 , wherein the test sample is from a specimen selected from the group consisting of a tissue specimen, a biopsy specimen, a surgical specimen, a cytological specimen, cervical scrapings, cervical smear, cervical washing, vaginal excretions, and blood.
7. A method comprising:
(a) treating DNA isolated from cervical cells with a chemical reagent that selectively modifies a non-methylated cytosine residue relative to a methylated cytosine residue; and
(b) detecting the treated DNA, wherein detecting the treated DNA comprises:
(i) amplifying the treated DNA with a pair of primers to form an amplification product comprising the nucleotide sequence of SEQ ID NO:309; and
(ii) detecting the amplification product.
8. The method of claim 7 , wherein the product is detected by a method selected from the group consisting of electrophoresis, hybridization, amplification, sequencing, ligase chain reaction, chromatography, mass spectrometry, and combinations thereof.
9. The method of claim 7 , wherein the chemical reagent comprises bisulfite ions.
10. The method of claim 9 , wherein the method further comprises contacting the DNA in the test sample with alkali after contacting the DNA in the test sample with bisulfite ions.
11. The method of claim 7 , wherein the test sample comprises squamous cells, nucleic acids from squamous cells, adenocarcinoma cells, nucleic acids from adenocarcinoma cells, adenosquamous cell carcinoma cells, nucleic acids from adenosquamous carcinoma cells, or any combination thereof.
12. The method of claim 7 , wherein the test sample is from a specimen selected from the group consisting of a tissue specimen, a biopsy specimen, a surgical specimen, a cytological specimen, cervical scrapings, cervical smear, cervical washing, vaginal excretions, and blood.