Regenerative materials
Methods of making tissue fillers are provided. In certain embodiments, the tissue is flake-like and has regenerative properties.
1. A tissue matrix composition comprising:
a group of small, irregularly sized and flake-shaped grated tissue matrix fragments intertwined and interlocked with one another to form a foam with interconnected macropores able to transduce pressure,
wherein the group of flake-shaped tissue matrix fragments has a size distribution ranging from 0.2-5 mm in length, 0.2-3 mm in width, and 0.02-0.3 mm in thickness.
2. The composition of claim 1 , wherein the tissue matrix fragments comprise acellular tissue matrix.
3. The composition of claim 2 , wherein the acellular tissue matrix comprises an acellular dermal matrix.
4. The composition of claim 2 , wherein the acellular tissue matrix comprises an acellular porcine dermal matrix.
5. The composition of claim 1 , wherein the tissue matrix fragments are created using a process comprising freezing the tissue matrix prior to cutting grating.
6. The composition of claim 5 , wherein the tissue matrix fragments are created using a process comprising applying a cryoprotectant solution prior to freezing.
7. The composition of claim 6 , wherein the cryoprotectant solution comprises at least one of a maltodextrin, sucrose, polyethylene glycol (PEG), or polyvinylpyrrolidone (PVP) solution, or a combination thereof.
8. The composition of claim 7 , wherein the cryoprotectant solution comprises a maltodextrin solution.
9. The composition of claim 8 , wherein the maltodextrin solution comprises 5-50% (w/v) maltodextrin.
10. The composition of claim 8 , wherein the maltodextrin solution comprises 15-25% (w/v) maltodextrin.
11. The composition of claim 1 , wherein the tissue matrix is grated with a grater.
12. The composition of claim 1 , wherein the tissue matrix is grated with a grating wheel.
13. The composition of claim 1 , wherein the group of tissue matrix fragments have been sterilized by application of at least one of ethylene oxide, propylene oxide, gamma irradiation or e-beam irradiation.
14. The composition of claim 1 , further comprising a bioactive substance.
15. The composition of claim 14 , wherein the bioactive substance comprises an antimicrobial agent.
16. The composition of claim 14 , wherein the bioactive substance comprises a cytokine.
17. The composition of claim 14 , wherein the bioactive substance comprises a growth factor.
18. The composition of claim 14 , wherein the bioactive substance comprises non-collagenous tissue.
19. The composition of claim 18 , wherein the non-collagenous tissue comprises adipose tissue.
20. The composition of claim 14 , wherein the bioactive substance comprises cells.
21. The composition of claim 20 , wherein the cells comprise stem cells.
22. The composition of claim 1 , wherein the group of flake-shaped tissue matrix fragments have been formed into the foam by placing the fragment in a liquid to form a stable suspension without gelling or phase separation of interconnected irregularly shaped particles and drying the suspension.
23. The composition of claim 22 , wherein drying the suspension comprises freeze-drying the suspension.