Lighting systems and methods of using lighting systems for in virto potency assay for photofrin
Presently disclosed is a lighting system and methods of using the lighting system for in vitro potency assay for photofrin. The lighting system includes a lamp housing, a first lens, an infrared absorbing filter, an optical filter, and a second lens. The lamp housing includes a lamp and a light-port. In operation, broad spectrum light from the lamp exits the lamp housing by passing through the light-port. The first lens then collimates the broad spectrum light that exits the lamp housing through the light-port. The infrared absorbing filter then passes a first portion of the collimated broad spectrum light to the optical filter and absorbs infrared light of the broad spectrum light. The optical filter then passes a second portion of the collimated broad spectrum light to the second lens. The second lens then disperses the second portion of the collimated light to provide uniform irradiation of a cell culture plate. A method of using the lighting system for studying a photosensitizer is also disclosed.
1. A lighting system comprising:
a lamp housing including a lamp and a light-port, wherein broad spectrum light from the lamp exits the lamp housing through the light-port;
a first lens to collimate the broad spectrum light that exits the lamp housing through the light-port;
an infrared absorbing filter to pass a first portion of the collimated broad spectrum light and absorb infrared light of the broad spectrum light that passes through the light-port and that is collimated by the first lens;
a reflector to reflect the first portion of the collimated broad spectrum light that passes through the infrared absorbing filter;
a short pass filter to pass a second portion of the collimated broad spectrum light, wherein the second portion of the collimated broad spectrum light comprises a subset of the first portion of the collimated broad spectrum light, and wherein the short pass filter filters out light of the first portion of the collimated broad spectrum light of wavelengths greater than 650 nm and less than 595 nm;
an infrared blocking filter to pass the second portion of the collimated broad spectrum light after passing through the short pass filter, wherein the infrared blocking filter absorbs residual infrared light of the first portion of the collimated broad spectrum light; and
a second lens to disperse the second portion of the collimated light that passed through the infrared blocking filter and the short pass filter.
2. A method for studying a photosensitizer, the method comprising:
adding the photosensitizer to a portion of wells on a cell culture plate to form photosensitizer assay wells, the wells comprising carcinoma cells;
incubating the photosensitizer assay wells for a first predetermined time period;
optionally washing the photosensitizer assay wells;
irradiating the photosensitizer assay wells with the lighting system of claim 1 at a predetermined wavelength to form irradiated wells, wherein each well is uniformly irradiated;
incubating the irradiated wells for a second predetermined time period; and
determining percent viability of the carcinoma cells contained in the wells.
3. The method according to claim 2 , wherein the photosensitizer is a porphyrin-based anti-neoplastic agent.
4. The method according to claim 3 , wherein the porphyrin-based anti-neoplastic agent is porfimer sodium.
5. The method according to claim 2 , wherein the photosensitizer assay wells are irradiated with light within a range of 400 nm to 650 nm.
6. The method according to claim 2 , wherein the carcinoma cells are A549 human lung carcinoma cells.
7. The method according to claim 2 , wherein the cell culture plate is an opaque black plate.
8. The lighting system of claim 1 , further comprising:
a base positioned on a surface below the second lens;
a ring stand coupled to the base; and
a first support ring removably attached to the ring stand.
9. The lighting system of claim 8 ,
wherein the second lens comprises a first dispersing lens and a second dispersing lens; and
wherein the first support ring holds the first dispersing lens and the second dispersing lens in place.
10. The lighting system of claim 9 ,
wherein the first dispersing lens comprises a first plano convex lens;
wherein the second dispersing lens comprises a second plano convex lens;
wherein the first plano convex lens comprises a first plano side and a first convex side;
wherein the second plano convex lens comprises a second plano side and a second convex side; and
wherein the first convex side is adjacent to the second convex side with a gap between the first convex side and the second convex side.
11. The lighting system of claim 1 , further comprising:
a wall,
wherein the light-port is located within the wall, and
wherein a position of the second lens is adjustable in at least one of a direction parallel to the wall and a direction perpendicular to the wall.
12. The lighting system of claim 11 , further comprising:
a shelf, and
a lens slider including a first hole for passing light;
wherein the shelf comprises a shelf riser parallel to the wall,
wherein the shelf comprises a shelf top perpendicular to the wall,
wherein the shelf top includes a second hole for passing light,
wherein the second lens is removably attached to the lens slider,
wherein the lens slider is removably attached to the shelf top, and
wherein at least a portion of the first hole is above or below at least a portion of the second hole.
13. A method comprising:
using the lighting system of claim 1 to irradiate contents of a cell culture plate.
14. The lighting system of claim 1 , wherein the first lens comprises a condenser lens or a Fresnel lens.
15. The lighting system of claim 1 , wherein the reflector comprises a dichroic mirror.
16. The lighting system of claim 1 , wherein the lamp housing is sealed so that less than 1% of the broad spectrum light from the lamp exits the lamp housing other than through the light-port.
17. The lighting system of claim 1 ,
wherein the lamp housing comprises a top,
wherein the light-port is within the top,
wherein the first lens is within the light-port, and
wherein the infrared absorbing filter, the infrared blocking filter, the short pass filter, and the second lens are located at positions above the top.
18. The lighting system of claim 1 ,
wherein the lamp housing comprises a bottom,
wherein the light-port is within the bottom,
wherein the first lens is within the light-port, and
wherein the infrared absorbing filter, the infrared blocking filter, the short pass filter, and the second lens are located at positions below the bottom.
19. The lighting system of claim 1 , wherein the collimated broad spectrum light has a spectral range of 400 nm to 700 nm, and wherein the second portion of the collimated broad spectrum light has a spectral range of 595 nm to 645 nm.