IP Library Granted Patent US 9,996,778
Granted Patent B2
US 9,996,778 · App. 15/175,430 · Granted Jun 12, 2018

Methods of storing information using nucleic acids

Inventor: George M. Church (Brookline, MA)
Assignee: President and Fellows of Harvard College
G06K19/022G06F19/22G06K19/00G06N3/123
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Quick Facts
Patent No.
US 9,996,778
App. No.
15/175,430
Granted
Jun 12, 2018
Kind
B2
Abstract

The present invention relates to methods of storing data using one or more nucleic acids.

Claims (11)

1. A method of storing text or an image using nucleotides comprising

converting the text or the image into a plurality of bit sequences of a bit stream,

converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, wherein the base is a member of a base pair selected from the group consisting of

synthesizing the plurality of corresponding oligonucleotide sequences using a template-independent polymerase, and

storing the synthesized plurality of corresponding oligonucleotide sequences.

2. The method of claim 1 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

3. The method of claim 1 wherein the template-independent polymerase is terminal deoxynucleotidyl transferase or poly(A) polymerase.

4. The method of claim 1 wherein the oligonucleotide sequences are stored on a solid or semi-solid support.

5. The method of claim 1 wherein the oligonucleotide sequences are stored on a support selected from the group consisting of a slide, bead, chip, particle, strand, gel, sheet, tubing, sphere, container, capillary, pad, slice, film, and plate.

6. The method of claim 1 wherein the A or C encodes for a first bit and G or T encodes for a second bit.

7. The method of claim 1 wherein the A or C encodes for zero and G or T encodes for 1.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 13, 2016
From: CHURCH, GEORGE M.
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 039426/0772 →
Continuity (4)
Continuation 14415014
Provisional Application 61676081 · Jul 26, 2012
Provisional Application 61673690 · Jul 19, 2012
Related Publication 20160358055A1 · Dec 8, 2016