Solid medium for the storage of biological material
This invention relates to flat solid media for the storage of samples of biological materials and methods of analyzing biomolecules contained within the samples following storage. In particular, the invention relates to the storage and further analysis of biomolecules present in the biological materials, such as proteins, enzymes and nucleic acids. The invention finds particular utility in the dry, room temperature storage of biological materials.
1. A solid support for storing a biological material thereon, wherein the support comprises a cellulose matrix having sorbed thereto an alginate, a protein denaturing reagent, and a free radical trap.
2. A solid support for storing a biological material thereon, wherein the support comprises a cellulose matrix having sorbed thereto an alginate, a protein denaturing reagent, and a chelating agent.
3. The support of claim 1 , further comprising a chaotrophic salt.
4. The support of claim 1 , wherein the alginate has antimicrobial activity.
5. The support of claim 1 , wherein the alginate is selected from the group consisting of calcium alginate, sodium alginate, potassium alginate, ammonium alginate, magnesium alginate, lithium alginate, and silver alginate.
6. A solid support for storing a biological material thereon, wherein the support comprises a cellulose matrix having sorbed thereto an alginate and a protein denaturing reagent, wherein the alginate is silver alginate.
7. The support of claim 1 , wherein the matrix is a solid matrix.
8. The support of claim 1 , wherein the biological material is stored thereon, and wherein the biological material is selected from the group consisting of a eukaryotic cell, a prokaryotic cell, and a prion cell.
9. The support of claim 1 , wherein the biological material is stored thereon, and wherein the biological material is selected from the group consisting of a blood, a plasma, a saliva, a urine and a buccal cell.
10. The support of claim 1 , wherein the biological material is stored thereon, and wherein the biological material comprises a biomolecule selected from the group consisting of a nucleic acid, a protein, a biopharmaceutical, and a polysaccharide.
11. A method for storing a biological material on a solid support comprising:
applying a biological material to a solid support comprising a cellulose matrix having sorbed thereto an alginate and a protein denaturing reagent, and
storing the biological material on the support without refrigeration.
12. The method of claim 11 , wherein the biological material is stored on the support at a temperature ranging from 4° C. to 50° C.
13. The method of claim 11 , wherein the sample is stored on the support for at least one day.
14. A method of detecting a biological material stored on a solid matrix support having sorbed thereto or incorporated therein an alginate and a protein denaturing reagent, the method comprising:
eluting the biological material from the support and detecting a presence, level, or both, of a biomolecule in the biological material.
15. The support of claim 1 , further comprising a chelating agent.
16. The support of claim 2 , further comprising a free radical trap and/or a chaotrophic salt.
17. The support of claim 2 , wherein the alginate is selected from the group consisting of calcium alginate, sodium alginate, potassium alginate, ammonium alginate, magnesium alginate, lithium alginate, and silver alginate.
18. The support of claim 2 , wherein the alginate is silver alginate.
19. The support of claim 2 , wherein the matrix is a solid matrix.
20. The support of claim 6 , further comprising a chelating agent, a free radical trap and/or a chaotrophic salt.
21. The support of claim 6 , wherein the matrix is a solid matrix.
22. The method of claim 11 , wherein the matrix has further sorbed thereto a chelating agent, a free radical trap and/or a chaotrophic salt.
23. The method of claim 11 , wherein the alginate is selected from the group consisting of calcium alginate, sodium alginate, potassium alginate, ammonium alginate, magnesium alginate, lithium alginate, and silver alginate.
24. The method of claim 11 , wherein the alginate is silver alginate.
25. The method of claim 14 , wherein the matrix has further sorbed thereto a chelating agent, a free radical trap and/or a chaotrophic salt.
26. The method of claim 14 , wherein the alginate is selected from the group consisting of calcium alginate, sodium alginate, potassium alginate, ammonium alginate, magnesium alginate, lithium alginate, and silver alginate.
27. The method of claim 14 , wherein the alginate is silver alginate.