Modified glucosyltransferases for producing branched alpha-glucan polymers
Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
1. A glucosyltransferase enzyme comprising an amino acid sequence that is at least 97% identical to
SEQ ID NO:87,
SEQ ID NO:91,
SEQ ID NO:95, or
SEQ ID NO:97,
wherein said glucosyltransferase enzyme lacks
the amino acid sequence of SEQ ID NO:79, and
wherein said glucosyltransferase enzyme produces a branched alpha-glucan polymer.
2. A reaction solution comprising water, sucrose, and a glucosyltransferase enzyme according to claim 1 .
3. A method for producing a branched alpha-glucan polymer comprising:
(a) contacting at least water, sucrose, and a glucosyltransferase enzyme according to claim 1 , whereby branched alpha-glucan polymer is produced, and
b) optionally, isolating the branched alpha-glucan polymer produced in step (a).
4. The glucosyltransferase of claim 1 , wherein the glucosyltransferase comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:87, SEQ ID NO:91, SEQ ID NO:95, or SEQ ID NO:97.