Method for preparing a polymeric reagent
A method for preparing a polymeric reagent is provided, which method includes the optional step of forming an N-hydroxysuccinimdyl ester derivative. Related methods, preparations, and so forth are also provided.
1. A method comprising:
reacting
with N,N-disuccinimydyl carbonate to result in
reacting mPEG-NH 2 with
to result in
subjecting
to base hydrolysis to result in
and, optionally,
reacting
with N-hydroxysuccinimide to result in
wherein, in each instance, mPEG- represents a poly(ethylene glycol) having a molecular weight of from about 100 Daltons to about 100,000 Daltons terminally capped with methoxy.
2. The method of claim 1 , comprising the optional step of reacting
with N-hydroxysuccinimide to result in
3. The method of claim 1 , wherein the poly(ethylene glycol) has a molecular weight of from about 1,000 Daltons to about 60,000 Daltons.
4. The method of claim 1 , wherein the poly(ethylene glycol) has a molecular weight of from about 2,000 Daltons to about 50,000 Daltons.
5. The method of claim 1 , wherein the poly(ethylene glycol) has a molecular weight of from about 5,000 Daltons.
6. The method of claim 1 , wherein the poly(ethylene glycol) has a molecular weight of from about 10,000 Daltons.
7. The method of claim 1 , wherein the poly(ethylene glycol) has a molecular weight of from about 20,000 Daltons.