Measurement and uses of oxidative status
The present invention provides a method of determining the overall oxidative status of a body fluid or a tissue of a patient by measuring the oxidation-reduction potential (ORP) of the body fluid or tissue. The method has been found to be useful in the diagnosis, evaluation and monitoring of patients who have suffered a trauma (such as a head injury), patients suspected of being critically-ill or who are critically ill, patients who have an infection, and patients suspected of having a myocardial infarction (MI) or who have had an MI. The method has also been found useful in monitoring and evaluating exercise performance in patients. In addition, the method has been found useful in monitoring and evaluating stored blood products and patients who will receive such a product.
1. A method of safely transfusing an individual at risk for side effects resulting from transfusion with a blood product, comprising:
a) identifying the individual as being at risk for side effects resulting from transfusion with a blood product, using a method comprising:
i) determining the oxidation-reduction potential (ORP) value of body fluid from an individual to be transfused, and, comparing the determined ORP value with the ORP value of a corresponding body fluid from a normal subject; and,
ii) identifying the individual as being at risk for transfusion-related side effects, when the determined ORP value is significantly higher than the ORP value of the body fluid from the normal subject; and,
b) transfusing the individual with a blood product containing a level of oxidative species that is lower than the level of oxidant species in the body fluid from the individual.
2. The method of claim 1 , wherein the body fluid is whole blood.
3. The method of claim 1 , wherein the body fluid is packed red blood cells.
4. The method of claim 1 , wherein the ORP value is measured by contacting the body fluid with an electrode connected to a machine that provides a readout of the ORP.
5. The method of claim 1 , wherein the body fluid is selected from the group consisting of blood, urine, saliva, cerebrospinal fluid, tears, semen, vaginal secretions, amniotic fluid, and cord blood.
6. The method of claim 1 , wherein the body fluid is serum.