PRRSV minor protein-containing recombinant viral vectors and methods of making and use thereof
The present invention encompasses recombinant porcine reproductive and respiratory syndrome virus (PRRSV) vaccines or compositions. In particular, the invention encompasses recombinant adenovirus vectors encoding and expressing PRRSV gp2, gp3, gp4, gp5a, gp5 and/or E antigens, proteins, epitopes or immunogens. Such vaccines or compositions can be used to protect animals from PRRSV.
1. A safe and effective immunological or vaccine composition comprising: one or more recombinant viral vectors, comprising one or more heterologous polynucleotides, encoding porcine reproductive and respiratory syndrome virus (PRRSV) gp2, gp3, gp4, and E antigens, polypeptides, or ectodomains.
2. The composition of claim 1 , further comprising a pharmaceutically or veterinarily acceptable carrier, and wherein the one or more vectors comprise a recombinant adenovirus 5 PRRSV (Ad5-PRRSV) vector, a recombinant baculovirus PRRSV vector, a recombinant porcine cytomegalovirus PRRSV vector or a recombinant poxvirus PRRSV vector.
3. The composition of claim 2 , wherein the one or more vectors comprise: an Ad5-PRRSV vector.
4. The composition of claim 3 , wherein the one or more vectors comprise a mixture of two vectors, a first vector expressing the gp2, gp3, and gp4 polypeptides, and a second vector expressing the E polypeptide.
5. The composition of claim 3 , wherein the polynucleotide encodes:
a. a PRRSV gp2 polypeptide having at least 90% sequence identity to SEQ ID NO: 1, 14, 34-39, 71, or 80-89;
b. a PRRSV gp3 polypeptide having at least 90% sequence identity to SEQ ID NO: 3, 16, 4.0-45, 73, or 90-99;
c. a PRRSV gp4 polypeptide having at least 90% sequence identity to SEQ ID NO: 5, 18, 46-51, 75, 77, or 100-109; and
d. a PRRSV E polypeptide having at least 90% sequence identity to SEQ ID NO: 7, 52-58, or 130-139.
6. The composition of claim 5 , wherein the recombinant PRRSV vector is an Ad5-PRRSV vector, which comprises a polynucleotide having:
a. at least 90% sequence identity to a sequence as set forth in SEQ ID NO: 2, 4, 6, 8, 9, 10, 11, 12, 13, 15, 17, 21-23, 30, 70, 72, 74, or 76; or
b. the sequence as set forth in SEQ ID NO: 2, 4, 6, 8, 9, 10, 11, 12, 13, 15, 17, 21-23, 30, 70, 72, 74, or 76.
7. The composition of claim 1 , wherein the composition or vaccine comprises one or two recombinant Ad5-PRRSV vectors.
8. The composition of claim 7 , wherein the recombinant Ad5-PRRSV vector expresses in vivo in an animal host wild type PRRSV gp2, gp3, gp4, and E polypeptides.
9. The composition of claim 8 , wherein the gp2 polypeptide is has the sequence as set forth in SEQ ID NO: 1; the gp3 polypeptide has the sequence as set forth in SEQ ID NO: 3; the gp4 polypeptide has the sequence as set forth in SEQ ID NO: 5; and the E polypeptide has the sequence as set forth in SEQ ID NO: 7.
10. A recombinant Ad5-PRRSV vector comprising, or consisting essentially of, PRRSV gp2, gp3, gp4, and E antigen(s), polypeptide(s), or ectodomain(s).
11. The recombinant Ad5-PRRSV vector of claim 10 , wherein the one or more polynucleotide encode(s):
a. a PRRSV gp2 polypeptide having at least 90% sequence identity to SEQ ID NO: 1, 14, 34-39, 71, or 80-89;
b. a PRRSV gp3 polypeptide having at least 90% sequence identity to SEQ ID NO: 3, 16, 40-45, 73, or 90-99;
c. a PRRSV gp4 polypeptide having at least 90% sequence identity to SEQ ID NO: 5, 18, 46-51, 75, 77, or 100-109; and
d. a PRRSV E polypeptide having at least 90% sequence identity to SEQ ID NO: 7, 52-58, or 130-139.
12. The recombinant Ad5-PRRSV vector of claim 10 , wherein the one or more polynucleotides encode:
a. a PRRSV gp2 polypeptide having the sequence as set forth in SEQ ID NO: 1, 14, 34-39, 71, or 80-89;
b. a PRRSV gp3 polypeptide having the sequence as set forth in SEQ ID NO: 3, 16, 40-45, 73, or 90-99;
c. a PRRSV gp4 polypeptide having the sequence as set forth in SEQ ID NO: 5, 18, 46-51, 75, 77, or 100-109; and
d. a PRRSV E polypeptide having the sequence as set forth in SEQ ID NO: 7, 52-58, or 130-139.
13. The recombinant Ad5-PRRSV vector of claim 10 , wherein the Ad5-PRRSV vector comprises a polynucleotide encoding:
a. a PRRSV gp2 polypeptide having the sequence as set forth in SEQ ID NO: 1, 14, or 34-39;
b. a PRRSV gp3 polypeptide having the sequence as set forth in SEQ ID NO: 3, 16, or 40-45;
c. a PRRSV gp4 polypeptide having the sequence as set forth in SEQ ID NO: 5, 18, or 46-51; and
d. a PRRSV E polypeptide having the sequence as set forth in SEQ ID NO: 7, or 52-58.
14. The recombinant Ad5-PRRSV vector of claim 10 , wherein the Ad5-PRRSV vector comprises a polynucleotide encoding:
a. a PRRSV gp2 polypeptide having the sequence as set forth in SEQ ID NO: 1;
b. a PRRSV gp3 polypeptide having the sequence as set forth in SEQ ID NO: 3;
c. a PRRSV gp4 polypeptide having the sequence as set forth in SEQ ID NO: 5; and
d. a PRRSV E polypeptide having the sequence as set forth in SEQ ID NO: 7.
15. The recombinant Ad5-PRRSV vector of claim 10 , wherein the Ad5-PRRSV vector comprises a polynucleotide encoding PRRSV polypeptides having the sequences as set forth in SEQ ID NO: 1, 3, 5, and 7, and no other PRRSV polypeptides.
16. The recombinant Ad5-PRRSV vector of claim 10 , wherein the Ad5-PRRSV vector comprises polynucleotides comprising SEQ ID NO:21.
17. The recombinant Ad5-PRRSV vector of claim 10 , wherein the Ad5-PRRSV vector comprises polynucleotides comprising SEQ ID NO:22.
18. A method of vaccinating an animal in need thereof against PRRSV comprising administering to the animal the recombinant Ad5-PRRSV vector of claim 10 .
19. The method of claim 18 , wherein the Ad5-PRRSV vector comprises one or more polynucleotides encoding:
a. a PRRSV gp2 polypeptide having the sequence as set forth in SEQ ID NO: 1, 14, 34-39, 71, or 80-89;
b. a PRRSV gp3 polypeptide having the sequence as set forth in SEQ ID NO: 3, 16, 40-45, 73, or 90-99;
c. a PRRSV gp4 polypeptide having the sequence as set forth in SEQ ID NO: 5, 18, 46-51, 75, 77, or 100-109; and
d. a PRRSV E polypeptide having the sequence as set forth in SEQ ID NO: 7, 52-58, or 130-139.
20. The method of claim 18 , wherein the Ad5-PRRSV vector comprises one or more polynucleotides encoding:
a. a PRRSV gp2 polypeptide having the sequence as set forth in SEQ ID NO: 1;
b. a PRRSV gp3 polypeptide having the sequence as set forth in SEQ ID NO: 3;
c. a PRRSV gp4 polypeptide having the sequence as set forth in SEQ ID NO: 5; and
d. a PRRSV E polypeptide having the sequence as set forth in SEQ ID NO: 7.