Heating assembly for a bioreactor and an associated method thereof

1. A method comprising:

receiving a cell-culture media comprising a plurality of cells in a culture vessel of a bioreactor;

heating the cell-culture media using a heating assembly coupled to the culture vessel, wherein the heating assembly comprises:

a holder comprising a plurality of segments coupled to each other to

define a unitary structure, wherein the unitary structure comprises:

a top end;

a bottom end; and

a side wall extending between the top end and the bottom end and along a circumferential direction of the heating assembly to define a cavity, wherein at least a portion of the unitary structure has a gradually varied perimeter along a direction perpendicular to a plane which intersects the side wall and parallel to the top end and the bottom end; and

a heating component coupled to at least one segment of the plurality of segments; and

establishing and maintaining a temperature gradient along the side wall from the top end to the bottom end using the heating assembly; and

culturing the plurality of cells within the culture vessel at a constant temperature of the cell-culture media while the cell-culture media is exposed to the temperature gradient.

2. The method of claim 1 , further comprising controlling heating of at least one segment by the heating component via a controller based on a plurality of inputs signals received from a temperature sensor, wherein the temperature sensor is coupled to the unitary structure, and wherein the controller is coupled to the heating component and the temperature sensor.

3. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises heating the at least one segment comprising the top end, having a uniform perimeter, wherein the heating component is disposed on an outer peripheral surface portion of at least one segment and extends along the circumferential direction of the heating assembly.

4. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying a thermal resistance of the unitary structure along an axial direction of the heating assembly, wherein the side wall of the unitary structure has a gradually varied thickness from the top end to the bottom end.

5. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying a thermal resistance of the unitary structure along an axial direction of the heating assembly, wherein the side wall of the unitary structure has a gradually varied thermal resistance from the top end to the bottom end.

6. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by the heating component along an axial direction of the heating assembly, wherein the heating component has a gradually varied thickness from the top end to the bottom end.

7. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by the heating component along an axial direction of the heating assembly, wherein the heating component is disposed spirally around an outer peripheral surface of the side wall from the top end to the bottom end and wherein the heating component has a gradually varied electrical resistance.

8. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by the heating component along an axial direction of the heating assembly, wherein the heating component is disposed in a wave-like structure on an outer peripheral surface of the side wall from the top end to the bottom end, along the axial direction of the heating assembly, and wherein the heating component has a gradually varied electrical resistance.

9. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by the heating component along an axial direction of the heating assembly, wherein the heating component is disposed in a wave-like structure on an outer peripheral surface of the side wall from the top end to the bottom end, along an axial direction of the heating assembly, and wherein the heating component has a gradually varied width from the top end to the bottom end.

10. The method of claim 1 , wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by the heating component along an axial direction of the heating assembly, wherein the heating component is disposed in a wave-like structure on an outer peripheral surface of the side wall from the top end to the bottom end, along an axial direction of the heating assembly, and wherein the heating component comprises a plurality of turns separated from each other by a gap to form a plurality of gradually varied gaps from the top end to the bottom end.

11. The method of claim 1 , wherein the heating component comprises a plurality of heating components, wherein each heating component is disposed on a corresponding segment of the plurality of segments and extends along the circumferential direction or an axial direction of the heating assembly, wherein establishing and maintaining the temperature gradient comprises varying an amount of heat output by each of the plurality of heating components along the circumferential direction or the axial direction of the heating assembly, and wherein the plurality of heating components has a gradually varied electrical resistance along the axial direction of the heating assembly.

12. The method of claim 1 , wherein the heating component comprises a plurality of heating components, wherein each heating component is disposed on a corresponding segment of the plurality of segments and extends along the circumferential direction or an axial direction of the heating assembly, wherein establishing and maintaining the temperature gradient comprises supplying a first power to one heating component and a second power to another heating component, and wherein the second power is different from the first power.