Methods and apparati for nondestructive detection of undissolved particles in a fluid
The apparati, methods, and computer program products disclosed herein can be used to nondestructively detect undissolved particles, such as glass flakes and/or protein aggregates, in a fluid in a vessel, such as, but not limited to, a fluid that contains a drug.
1. A method for nondestructive detection of an undissolved particle in a vessel that is at least partially filled with a fluid, the method comprising:
(a) using at least one imager to image the particle;
(b) processing the image to determine position data indicative of a position of the particle in the vessel;
(c) detecting the particle based at least in part on the position data, wherein detecting the particle based at least in part on the position data comprises identifying the presence of the particle in a sub-region of the vessel;
(d) using a sensor to determine a characteristic of the particle when the particle is located in the sub-region of the vessel,
(e) generating particle characteristic data indicative of the determined characteristic; and
(f) associating the particle characteristic data with data identifying the particle.
2. The method of claim 1 , wherein:
the at least one imager comprises at least two imagers positioned to image the particle from different perspectives to each acquire a respective one or more two dimensional images of the particle in the fluid;
processing the image to determine position data comprises combining the two dimensional images from the at least two imagers to determine three dimensional data indicative of the position of the particle in the vessel; and
detecting the particle comprises detecting the particle based at least in part on the three dimensional data.
3. The method of claim 2 , wherein the characteristic of the particle comprises a spectral characteristic.
4. The method of claim 3 , wherein the sensor comprises a spectrometer device positioned to sense one or more spectral characteristics of light incident from the sub-region of the vessel.
5. The method of claim 4 , wherein the sub-region of the vessel comprises a substantially planar layer of fluid in the vessel.
6. The method of claim 5 , wherein the sensor comprises a telecentric imager positioned to image the sub-region of the vessel onto the spectrometer device.
7. The method of claim 4 , wherein the one or more spectral characteristics comprise a color, an absorption spectrum, or a transmission spectrum or a combination of any of these.
8. An apparatus for nondestructive detection of an undissolved particle in a vessel that is at least partially filled with a fluid, the apparatus comprising:
(a) at least one imager positioned to image the particle;
(b) at least one sensor configured to determine a characteristic of the particle when the particle is located in a sub-region of the vessel;
(c) at least one processer operably coupled to each of the at least one imager and the sensor and configured to:
process an image from the at least one imager to determine a position data indicative of the position of the particle in the vessel;
detect the particle based at least in part on the position data and identify a presence of the particle in the sub-region of the vessel;
use a signal from the sensor to determine a characteristic of the particle when the particle is located in the sub-region of the vessel;
generate particle characteristic data indicative of the determined characteristic; and
associate the particle characteristic data with data identifying the particle.
9. The apparatus of claim 8 , wherein:
the at least one imager comprises at least two imagers positioned to image the particle from different perspectives to each acquire a respective one or more two dimensional images of the particle in the fluid;
the processor is configured to combine the two dimensional images from the at least two imagers to determine three dimensional data indicative of the position of the particle in the vessel; and
the processor is configured to detect the particle based at least in part on the three dimensional data.
10. The apparatus of claim 9 , wherein the characteristic of the particle comprises a spectral characteristic.
11. The apparatus of claim 10 , wherein the sensor comprises a spectrometer device positioned to sense one or more spectral characteristics of light incident from the sub-region of the vessel.
12. The apparatus of claim 11 , wherein the sub region comprises a substantially planar layer of fluid in the vessel.
13. The apparatus of claim 12 , wherein the sensor comprises a telecentric imager positioned to image the sub-region of the vessel onto the spectrometer.
14. The apparatus of claim 11 , wherein the one or more spectral characteristics comprise a color, an absorption spectrum, or a transmission spectrum or a combination of any of these.