Method of reseeding adherent cells grown in a hollow fiber bioreactor system

1. A method for directly reseeding cells grown in growth media in a cell growth space of a hollow fiber bioreactor, the method comprising:

removing a first portion of the growth media from the cell growth space;

washing the cells to remove a second portion of the growth media from the cell growth space;

loosening the cells from the cell growth space by adding trypsin to the cell growth space;

before removing the cells from the cell growth space to a cell harvest bag, adding a harvest media to the cell harvest bag, wherein the harvest media comprises a protein and metabolites to provide an environment conducive to cell growth;

removing the cells and the trypsin from the cell growth space;

inactivating the removed trypsin;

adding the removed cells and the removed trypsin to the harvest media in the cell harvest bag; and

without additional washing, reseeding the removed cells.

2. The method of claim 1 , wherein the cell growth space comprises one of an intracapillary space or an extracapillary space.

3. The method of claim 2 , wherein the removing the cells comprises pushing fluid through hollow fibers from one of the intracapillary space or the extracapillary space that is not the cell growth space to the cell growth space.

4. The method of claim 1 , wherein the removed trypsin is inactivated with complete media.

5. The method of claim 1 , wherein the protein in the harvest media comprises one or more from the group consisting of: human platelet lysate, human plasma, fetal bovine serum, and fetal calf serum.

6. The method of claim 1 , wherein the adding the harvest media to the cell harvest bag comprises pumping the harvest media from a fluid container into the cell harvest bag.

7. The method of claim 1 , wherein the cells comprise adherent cells.

8. The method of claim 7 , wherein the adherent cells comprise mesenchymal stem cells.

9. The method of claim 1 , wherein the reseeding the removed cells comprises:

reseeding a portion of the removed cells.

10. The method of claim 1 , wherein the reseeding the removed cells comprises:

completely reseeding the removed cells.

11. The method of claim 1 , wherein the hollow fiber bioreactor comprises a plurality of hollow fibers.

12. The method of claim 11 , wherein the removing the cells comprises pushing fluid through the plurality of hollow fibers.

13. The method of claim 1 , wherein the cells are reseeded into the same hollow fiber bioreactor.

14. The method of claim 1 , wherein the cells are reseeded into a different hollow fiber bioreactor.

15. The method of claim 1 , wherein the metabolites comprise at least one of calcium chloride, anhydrous D-glucose, and/or magnesium sulfate.

16. The method of claim 1 , wherein the harvest media comprises base media.

17. The method of claim 16 , wherein the base media comprises alpha-MEM.